Anti-SUPT6H/SPT6 Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q7KZ85

Core Product Specifications and Parameters

Biological Background: SUPT6H (SPT6) Function and Localization

• Transcription elongation factor SPT6 (SPT6H) acts as a histone H3-H4 chaperone, facilitating RNA polymerase II transcription elongation and enhancing Tat-mediated activation of HIV-1 transcription.
• Forms a complex with IWS1 and the C-terminal domain (CTD) of RNAPII subunit RPB1, coupling transcription with mRNA processing, splicing, and export.
• Recruits mRNA export factors (ALYREF/THOC4, EXOSC10) and histone modifying enzymes (e.g., SETD2) to ensure proper mRNA maturation and deposition of elongation-coupled H3K36me3 marks.
• Associates with SETD1A to catalyze H3K4me3 at AICDA target loci, regulating immunoglobulin class-switch recombination and somatic hypermutation.
• Promotes muscle differentiation by stabilizing the chromatin interaction of H3K27 demethylase KDM6A, thereby erasing repressive H3K27me3 marks at myogenic genes.
• Localizes to the nucleus and is ubiquitously expressed across tissues.
• Additional features include multiple phosphorylation and acetylation sites, and production of alternatively spliced isoforms.

Experimental Guidance and Technical Tips

• For western blotting, given the large protein size (199 kDa), consider using a low-percentage acrylamide gel (e.g., 6-8%) and prolonged transfer times with an appropriate membrane.
• In immunoprecipitation and ELISA, the polyclonal nature may enhance capture efficiency, but we recommend titrating the antibody and validating specificity in the target sample system.
• For immunofluorescence/ICC, expect nuclear staining; include relevant controls and optimize fixation/permeabilization conditions.
• Because the antibody cross-reacts with mouse and rat, it may be suitable for studies across these species; however, individual validation in the chosen model is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined procurement pathway for IVD raw materials, and this SUPT6H antibody supports investigations into transcription elongation and chromatin dynamics. As a dedicated sourcing partner, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare-target raw materials to accelerate your diagnostic development from concept to clinic.