Anti-SUPT5H/SPT5 Rabbit Polyclonal Antibody for WB, IP, ELISA - O00267

Core Product Specifications and Parameters

Biological Background: SUPT5H/SPT5 Function and Localization

• SUPT5H/SPT5 encodes the large subunit (p160) of the DRB sensitivity-inducing factor (DSIF) complex, a transcription elongation factor that regulates RNA polymerase II processivity and mRNA processing. Related references: PMID:10075709 PMID:10199401 PMID:10421630
• DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A, thereby coupling elongation to 5′ cap formation. Related references: PMID:10075709 PMID:10421630 PMID:10757782
• Together with the negative elongation factor complex (NELF), DSIF enhances promoter-proximal transcriptional pausing, facilitating the assembly of an elongation-competent RNA polymerase II complex. Related references: PMID:10075709 PMID:10199401 PMID:10757782
• DSIF and NELF promote transcriptional pausing by inhibiting the elongation factor TFIIS/S-II, which stimulates RNA polymerase II intrinsic nuclease activity at pause sites. Related references: PMID:16214896
• Phosphorylation of DSIF by CDK9 converts the complex into a positive elongation factor, enabling productive transcription elongation. Related references: PMID:16427012
• SUPT5H/SPT5 is required for the efficient activation of transcriptional elongation by the HIV-1 Tat protein; it suppresses premature termination of HIV-1 transcripts and overcomes pausing at terminator sequences. Related references: PMID:10393184 PMID:10454543 PMID:11809800 PMID:11112772 PMID:14701750
• The protein localizes predominantly to the nucleus, is ubiquitously expressed, and undergoes extensive post-translational modifications including phosphorylation, acetylation, methylation, and ubiquitination, reflecting its dynamic regulation in transcription.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 125–425 within the mid-region of human SUPT5H. This region is conserved in mouse, supporting the observed cross-reactivity.
• For western blot (WB) analysis, a predominant band at approximately 121 kDa is expected. Consider using nuclear extracts to enrich endogenous target detection.
• Because SUPT5H forms a stable complex with SUPT4H (DSIF), co-immunoprecipitation (IP) experiments can be designed to study the DSIF complex and its associated factors.
• The antibody is also suitable for ELISA; however, optimal working conditions should be validated in the specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a streamlined, one-stop channel for IVD raw materials. This SUPT5H/SPT5 antibody supports research in transcriptional regulation, RNA polymerase II elongation, and HIV-1 Tat transactivation. Beyond polyclonal antibodies, our portfolio includes validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing — helping you move from discovery to diagnostic development with efficiency and confidence.