Anti-SUPT5H/SPT5 Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - O00267

Core Product Specifications and Parameters

Biological Background: SUPT5H/SPT5 Function and Localization

• SPT5 (SUPT5H) is the large subunit of the DRB sensitivity-inducing factor (DSIF) complex, which regulates mRNA processing and transcription elongation by RNA polymerase II.
  Related references: PMID:10075709, PMID:10199401, PMID:10421630

• DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A.
  Related references: PMID:10075709, PMID:10421630, PMID:10757782

• DSIF cooperates with the negative elongation factor (NELF) complex to enhance transcriptional pausing at promoter-proximal sites, facilitating the assembly of an elongation-competent RNA polymerase II complex.
  Related references: PMID:10075709, PMID:10199401, PMID:10757782

• DSIF and NELF promote pausing by inhibiting the transcription elongation factor TFIIS/S-II, which stimulates the intrinsic nuclease activity of RNA polymerase II to cleave blocked transcripts, promoting resumption of transcription.
  Related references: PMID:16214896

• Following phosphorylation by CDK9, DSIF can switch to a positive elongation factor, enabling productive transcriptional elongation.
  Related references: PMID:16427012

• SPT5 is essential for HIV-1 Tat-mediated transcriptional activation, suppressing pausing and preventing premature termination of viral transcripts.
  Related references: PMID:10393184, PMID:10454543, PMID:11809800, PMID:11112772, PMID:14701750

• SPT5 localizes predominantly to the nucleus and is ubiquitously expressed across tissues.

• SPT5 undergoes multiple post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination, reflecting its dynamic regulation in transcription.

Experimental Guidance and Technical Tips

• The immunogen was designed from a synthetic peptide within amino acids 800–900 of human SPT5 (C-terminal region). This region is likely conserved across human, mouse, and rat, consistent with the reported cross-reactivity.
• For Western blotting (WB), endogenous SPT5 is detected at approximately 121 kDa. Consider using nuclear-enriched lysates to improve signal, given its nuclear localization.
• In immunoprecipitation (IP) assays, antibody performance may depend on the accessibility of the epitope; validate in the relevant sample system.
• For immunocytochemistry (IF/ICC) and immunohistochemistry (IHC-P), anticipate nuclear staining. Adequate permeabilization and antigen retrieval procedures may be necessary for optimal results.
• For ELISA applications, pairing with capture/detection antibodies targeting non-overlapping epitopes is recommended to ensure specificity.
• Titrate the antibody in your specific assay conditions, as optimal working concentrations may vary depending on sample type and detection method.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined, one-stop supply channel for IVD raw materials, technical services, and consulting—supporting projects from concept to clinic. For transcription elongation research targeting SPT5/DSIF, we can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare or specialized targets. Rely on CamelBio as your dedicated B2B partner to accelerate assay development and ensure consistent material quality.