Anti-SUPT20H Polyclonal Antibody for WB, IHC-P, ELISA - Q8NEM7

Core Product Specifications and Parameters

Biological Background: SUPT20H Function and Localization

• SUPT20H (suppressor of Ty 20 homolog) is a transcription factor homolog, also known as p38IP, FAM48A, and C13orf19, encoded by the SUPT20H gene on chromosome 13. Multiple isoforms exist due to alternative splicing.
• It plays an essential role in MAP kinase p38 (MAPK11, MAPK12, MAPK13, and/or MAPK14) activation during gastrulation, and is required for down-regulation of E-cadherin by regulating protein levels downstream of NCK-interacting kinase (NIK).
• SUPT20H functions independently of transcriptional regulation by FGF signaling and Snail, indicating a post-translational mechanism for E-cadherin control.
• This protein is involved in starvation-induced autophagy, being required for ATG9A trafficking, a key step in autophagosome formation.
• Localized to the nucleus, SUPT20H is highly expressed in testis, moderately in brain and pituitary gland, and in fetal tissues including lung, brain, thymus, and kidney; expression is down-regulated in malignant prostate tissues.
• Post-translational modifications include phosphorylation, and the protein has been identified in various proteomics studies.
• The predicted molecular weight is approximately 86 kDa (UniProt: 85,789 Da), consistent with the product's reported band at 86 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 580–779 of human SUPT20H (NP_001014308.2). This C-terminal region is likely to have good immunogenicity; consider verifying specificity with appropriate controls like siRNA knockdown or knockout lysates.
• For Western blot, a band around 86 kDa is expected. Use tissues with high expression such as testis or brain as positive samples, and consider a negative control tissue like prostate cancer where expression is down-regulated.
• In immunohistochemistry (IHC-P), testis or brain sections are recommended for initial optimization. Antigen retrieval conditions may need to be tailored to the epitope.
• The antibody's reactivity with human, mouse, and rat species suggests conserved epitope regions; validate species-specific detection by running parallel samples if cross-species use is intended.

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