Anti-SUPT16H (SPT16) Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y5B9

Core Product Specifications and Parameters

Biological Background: SUPT16H (SPT16) Function and Localization

• SUPT16H (SPT16) is a subunit of the FACT (Facilitates Chromatin Transcription) complex, a general chromatin factor that reorganizes nucleosomes to facilitate DNA-templated processes such as transcription elongation, DNA replication, and DNA repair.
• During transcription elongation, FACT acts as a histone chaperone, destabilizing nucleosomes by promoting the dissociation of one histone H2A-H2B dimer, thereby enabling RNA polymerase II passage, and then restoring nucleosome structure afterward.
• The FACT complex is also implicated in the phosphorylation of p53/TP53 at Ser-392 via its association with casein kinase II (CK2).
• SUPT16H is localized to the nucleus and associates with chromosomes during active DNA transactions.
• The protein is ubiquitously expressed across tissues.
• Post-translational modifications include acetylation, ADP-ribosylation, phosphorylation, and ubiquitylation (isopeptide bond formation).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 520-695) of human SUPT16H; consider verifying antibody specificity against full-length protein in your sample system.
• For Western blot, the predicted molecular weight is ~120 kDa; use a protein ladder with appropriate range and optimize transfer conditions for high molecular weight proteins.
• For immunofluorescence/immunocytochemistry, validate fixation and permeabilization protocols to ensure nuclear antigen accessibility.
• Cross-reactivity with mouse and rat has been demonstrated; if using other species, check sequence homology before testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw materials for IVD manufacturers and research laboratories. The SUPT16H (SPT16) antibody targets a key chromatin regulator, supporting research in epigenetics and cancer biology. Partner with CamelBio for reliable sourcing from concept to clinic.