Anti-SUN2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9UH99

Core Product Specifications and Parameters

Biological Background: SUN2 Function and Localization

• SUN2 is a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, mediating connections between the nuclear lamina and the cytoskeleton, which is critical for nuclear positioning and mechanotransduction.
• Together with SYNE2/nesprin-2, SUN2 forms transmembrane actin-associated nuclear (TAN) lines that couple the nucleus to retrograde actin flow, facilitating actin-dependent nuclear movement.
• SUN2 is required for interkinetic nuclear migration (INM) and nucleokinesis during radial neuronal and glial migration in the cerebral cortex, associating with cytoplasmic dynein-dynactin and kinesin motor complexes.
• Meiotically, SUN2 functions redundantly with SUN1 in the SUN1/2:KASH5 LINC complex to couple telomeres to microtubules, anchor chromosome movement in prophase, and is essential for telomere attachment to the nuclear envelope and gametogenesis.
• SUN2 localizes to the inner nuclear membrane, nuclear envelope, and endosome membrane; on endosomes, it may serve as a receptor for RAB5-GDP, participating in RAB5 activation.
• The protein is widely expressed, with high levels in heart, lung, and muscle, and shows slight overexpression in heart tissues from some congenital heart defect patients.
• UniProt annotations highlight glycosylation, phosphorylation, disulfide bond formation, and a transmembrane helix as key structural features.

Experimental Guidance and Technical Tips

• The immunogen spans the coiled-coil and SUN domain regions (a.a. 255–572), which may generate polyclonal reactivity against multiple epitopes; consider validating specificity in knockout or knockdown systems if feasible.
• For Western blotting, expect a band near 80 kDa; optimize blocking and detection conditions to reduce nonspecific binding typical with polyclonal antibodies.
• In IHC-P, test antigen retrieval protocols (e.g., citrate or EDTA buffer) to unmask nuclear envelope epitopes; include appropriate tissue controls with known SUN2 expression (heart, lung, muscle).
• For IF/ICC, gentle permeabilization (e.g., 0.1% Triton X-100) may help preserve nuclear membrane integrity; co-staining with lamin or nesprin markers can confirm localization.
• ELISA can be used for antibody titration or antigen detection; consider a direct or sandwich format with recombinant SUN2 fragments for quantitative assays.
• Given cross-reactivity with mouse and rat, verify reactivity in the desired species and tissue context before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents to support nuclear envelope and cytoskeletal research. This anti-SUN2 antibody, targeting a key LINC complex protein, is available along with bulk ordering options and technical consultation to streamline your assay development. For rare target raw-material needs or customized solutions in cell migration and meiosis studies, CamelBio’s sourcing network provides flexible and reliable supply chains.