Anti-SUN2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9UH99

Core Product Specifications and Parameters

Biological Background: SUN2 Function and Localization

• SUN2, also known as protein unc-84 homolog B, Rab5IP, and Sad1/unc-84 protein-like 2, is encoded by the SUN2 gene and exists in multiple isoforms through alternative splicing.
• As a core component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, SUN2 bridges the nuclear lamina and the cytoskeleton, transmitting mechanical forces across the nuclear envelope and governing nuclear positioning and movement.
• SUN2 collaborates with SYNE2 to form transmembrane actin-associated nuclear (TAN) lines that couple the nucleus to retrograde F-actin flow, essential for actin-dependent nuclear migration.
• During development, SUN2 is required for interkinetic nuclear migration (INM) and radial neuronal and glial migration in the cerebral cortex, as well as nuclear migration in retinal photoreceptor progenitors via dynein-dynactin and kinesin motors.
• Together with SUN1, SUN2 mediates telomere attachment to the nuclear envelope during meiosis, anchoring chromosome movement in prophase and supporting gametogenesis-selective gene expression.
• Beyond the nuclear envelope, SUN2 localizes to endosome membranes, where it may act as a receptor for RAB5-GDP and participate in RAB5 activation on endocytic vesicles.
• SUN2 undergoes post-translational modifications including glycosylation, phosphorylation, and disulfide bond formation, and its subcellular distribution spans the inner nuclear membrane, nuclear envelope, and endosome.
• Widely expressed with highest levels in heart, lung, and muscle, SUN2 shows slight overexpression in some heart tissues from patients with congenital heart defects.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 618–717 of human SUN2. For western blot (WB), expect a band near 80 kDa; use appropriate positive controls (e.g., HeLa, HEK293, or heart tissue lysates) and validate in the relevant sample system.
• For immunohistochemistry-paraffin (IHC-P), consider antigen retrieval optimization and include tissue sections known to express SUN2 (heart, muscle, or lung) along with negative controls to confirm specificity.
• The antibody cross-reacts with mouse and rat SUN2, enabling translational studies across species; verify cross-reactivity in your experimental model before large-scale use.
• As a rabbit monoclonal, this reagent provides consistent lot-to-lot performance, supporting reproducible quantitative ELISA and multiplexed detection schemes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material sourcing for diagnostic and research institutions, offering validated antibody reagents like this SUN2 monoclonal antibody for nuclear migration and LINC complex studies. Our supply network provides monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target procurement to support your assay development from discovery to validation.