Anti-SUMO4 Polyclonal Antibody for WB, IHC-P, ELISA - Q6EEV6

Core Product Specifications and Parameters

Biological Background: SUMO4 Function and Localization

• SUMO4 (Small ubiquitin-related modifier 4) is a ubiquitin-like protein that can be covalently attached to target lysines as a monomer.
• Unlike ubiquitin, SUMO4 conjugation does not lead to protein degradation; instead, it modulates substrate subcellular localization, stability, or activity.
• Upon oxidative stress, SUMO4 conjugates to various anti-oxidant enzymes, chaperones, and stress defense proteins, suggesting a role in cellular stress response.
• SUMO4 negatively regulates the transcriptional activity of NFKBIA, TFAP2A, and FOS, while positively regulating NR3C1 transcriptional activity.
• Covalent attachment to substrates requires activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, forming an isopeptide bond.
• Tissue expression is mainly in adult and embryonic kidney, with highest levels in immune tissues such as lymph node and spleen.
• SUMO4 is associated with diabetes mellitus (IDDM5) and is part of the ubiquitin-like conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen is a peptide from residues 40-95, which may recognize both monomeric and conjugated SUMO4; consider assessing specificity for sumoylated versus free forms in your assay.
• For Western blot, SUMO4 has a predicted molecular weight of ~11 kDa, but covalent conjugates will migrate at higher molecular weights; use appropriate reducing conditions and validate with known sumoylation substrates.
• For immunohistochemistry (IHC-P), positive staining is expected in kidney and immune tissues; include appropriate negative controls to confirm specificity.
• For ELISA, optimize coating and detection conditions using purified SUMO4 or recombinant proteins as standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, including validated antibodies, bulk ancillary reagents, and rare target sourcing. Our SUMO4 antibody supports research into ubiquitin-like modifications, oxidative stress, and diabetes pathways. As your strategic partner, we streamline procurement from concept to clinic, ensuring reliable supply for assay development and validation.