Anti-SUMO3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P55854

Core Product Specifications and Parameters

Biological Background: SUMO3 Function and Localization

• SUMO3 (Small Ubiquitin-related Modifier 3) is a ubiquitin-like protein encoded by the SUMO3 gene (GeneID: 6612). It is a member of the SUMO family and is also known as SMT3A, Smt3B, SMT3H1, or SUMO-2.
• Unlike ubiquitin, SUMO3 primarily functions as a post-translational modifier that does not target proteins for degradation; instead, it may antagonize ubiquitin-dependent proteolysis. It is covalently attached to target lysines as a monomer or polymer.
• The conjugation process requires an E1 activating enzyme (SAE1-SAE2), an E2 conjugating enzyme (UBE2I), and is promoted by E3 ligases such as PIAS1-4, RANBP2, or CBX4. Related references: PMID:11451954, PMID:18538659, PMID:21965678
• SUMO3 participates in nuclear transport, DNA replication and repair, mitosis, and signal transduction. It is also critical for regulating the sumoylation of SETX. Related references: PMID:24105744
• Subcellularly, SUMO3 is found in the cytoplasm, nucleus, and PML nuclear bodies.
• It is predominantly expressed in the liver, with a predicted molecular weight of 11.6 kDa (migrating at ~12 kDa in SDS-PAGE).
• UniProt keywords include: 3D-structure, alternative splicing, isopeptide bond, proteomics identification, and Ubl conjugation pathway.

Experimental Guidance and Technical Tips

• Due to the small size of SUMO3 (~12 kDa), use 15% SDS-PAGE gels or gradient gels for optimal resolution in Western blot, and ensure efficient transfer onto PVDF membranes with low molecular weight cut-offs.
• For immunofluorescence (IF/ICC), fix cells with 4% paraformaldehyde and permeabilize with 0.1-0.2% Triton X-100 to allow antibody access to nuclear and PML body-localized SUMO3.
• Validate antibody specificity in relevant cell lines or tissues; SUMO3 is highly expressed in liver, so HepG2 or primary hepatocytes may serve as positive controls.
• Since this polyclonal antibody may recognize both free and conjugated forms, consider using 1% SDS or other denaturing conditions to reduce non-specific binding in ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with one-stop access to high-quality IVD raw materials, including this validated SUMO3 polyclonal antibody for the ubiquitin-like modification research field. We support your assay development from concept to clinic with validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets. Rely on CamelBio as your trusted partner in building robust detection platforms.