Anti-SUMO2/3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P61956/P55854

Core Product Specifications and Parameters

Biological Background: SUMO2/3 Function and Localization

• Small ubiquitin-related modifier 2 (SUMO2), also known as HSMT3, SMT3B, Sentrin-2, or Ubiquitin-like protein SMT3B, is a member of the SUMO family of ubiquitin-like proteins. It shares high sequence homology with SUMO3, and this antibody recognizes both SUMO2 and SUMO3 (SUMO2/3).
• SUMO2 functions as a ubiquitin-like protein that can be covalently attached to lysine residues of target substrates, either as a monomer or as a lysine-linked polymeric chain. This modification, termed SUMOylation, requires activation by the E1 complex SAE1-SAE2, conjugation by the E2 enzyme UBE2I, and often promotion by E3 ligases such as PIAS1-4, RANBP2, CBX4, or ZNF451. Related references: PMID:26524494
• Polymeric SUMO2 chains are susceptible to polyubiquitination, which serves as a signal for proteasomal degradation of the modified proteins. Related references: PMID:18408734, PMID:18538659, PMID:21965678
• SUMO2 plays a crucial role in various cellular processes, including nuclear transport, DNA replication and repair, mitosis, and signal transduction.
• It is involved in the regulation of sumoylation status of SETX. Related references: PMID:24105744
• Subcellularly, SUMO2 localizes predominantly to the nucleus and nuclear PML bodies.
• SUMO2 is broadly expressed across tissues.
• Keywords associated with SUMO2 include: 3D-structure, acetylation, alternative splicing, host-virus interaction, isopeptide bond, nucleus, proteomics identification, reference proteome, Ubl conjugation, Ubl conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 1-95 of human SUMO2/3, covering the full-length protein of ~11 kDa. When performing Western blot, expect a band around 11 kDa for free SUMO2/3, with additional higher molecular weight bands representing SUMOylated protein conjugates.
• For immunohistochemistry (IHC-P), consider standard antigen retrieval methods and validate the antibody in relevant tissue samples, as SUMO2 is broadly expressed but may show nuclear enrichment.
• In ELISA applications, use appropriate controls to confirm specificity for SUMO2/3, given the high homology between SUMO family members.
• As the antibody cross-reacts with human, mouse, and rat, it is suitable for studies across these species; however, confirm reactivity in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers, labs, and research institutes with comprehensive access to antibodies, recombinant proteins, and ancillary reagents—from concept to clinic. Our SUMO2/3 antibody supports investigations into SUMOylation pathways, ubiquitin-like modifications, and cellular stress responses. Beyond this antibody, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and tailored rare-target raw-material sourcing to streamline your assay development.