Anti-SUMO2/3 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P61956 / P55854

Core Product Specifications and Parameters

Biological Background: SUMO2/3 Function and Localization

• Small ubiquitin-related modifier 2 (SUMO2), also known as HSMT3, SMT3B, SUMO-3, Sentrin-2, is a ubiquitin-like protein that can be covalently attached to lysine residues of target proteins as a monomer or as a lysine-linked polymer.
• Covalent attachment of SUMO2 to substrates requires prior activation by the E1 complex SAE1-SAE2, linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2, CBX4 or ZNF451. Related references: PMID:26524494
• SUMO2 modification plays a crucial role in a number of cellular processes including nuclear transport, DNA replication and repair, mitosis, and signal transduction.
• Polymeric SUMO2 chains are susceptible to polyubiquitination, which functions as a signal for proteasomal degradation of modified proteins. Related references: PMID:18408734, PMID:18538659, PMID:21965678
• SUMO2 regulates the sumoylation status of SETX. Related references: PMID:24105744
• Predominantly localized in the nucleus, specifically within PML nuclear bodies.
• Broadly expressed across tissues.
• Post-translational modifications include acetylation and formation of isopeptide bonds; SUMO2 is also involved in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-95) of human SUMO2/3. Note that SUMO proteins are often conjugated to target proteins, so the antibody may detect free SUMO (~11 kDa) as well as higher molecular weight SUMOylated proteins in Western blot. Validate the band pattern in your specific sample system.
• For immunofluorescence, expect nuclear staining with possible punctate PML body localization. Optimize fixation and permeabilization conditions for your cell type.
• The antibody cross-reacts with human, mouse, and rat. Confirm reactivity and specificity in your target tissue or cell line before large-scale experiments.
• For ELISA, consider coating with recombinant SUMO2/3 and titrate the antibody to determine an optimal working dilution for your assay format.

CamelBio: Your One-Stop Sourcing Bridge

• As a specialized supplier of IVD raw materials, CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—covering every stage from concept to clinic. This anti-SUMO2/3 antibody supports research into SUMOylation pathways, DNA repair, and signal transduction. Contact us for tailored procurement solutions and expert technical consultation.