Anti-SUMO1 Rabbit Polyclonal Antibody (KO Validated) for WB, IHC-P, IF/ICC, ELISA - P63165

Core Product Specifications and Parameters

Biological Background: SUMO1 Function and Localization

• Encodes small ubiquitin-related modifier 1 (also known as GMP1, PIC1, SMT3C, UBL1), a ubiquitin-like protein that is covalently conjugated to target lysine residues.
• Conjugation requires the E1 activating complex SAE1-SAE2, the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2, or CBX4.
• SUMOylation regulates nuclear transport (e.g., targets RANGAP1 to the nuclear pore complex), DNA replication and repair, mitosis, and signal transduction.
• Modulates ion channel function: covalently attached to the voltage-gated potassium channel KCNB1 to alter gating characteristics (PubMed:19223394). Related references: PMID:19223394
• Polymeric SUMO1 chains can be polyubiquitinated, serving as a signal for proteasomal degradation of modified proteins.
• Plays a role in palate development by regulating a gene network; covalently modifies ZFHX3 (PubMed:24651376). Related references: PMID:24651376
• Localizes to the nucleus membrane, nuclear speckles, cytoplasm, PML bodies, and cell membrane; also detected in the nucleus.
• Post-translationally modified by acetylation and phosphorylation; subject to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to the N-terminal region (aa 1–100), which likely detects total SUMO1. For modification‑specific analysis, alternative antibodies should be considered.
• KO validation confirms specificity; when performing WB, include appropriate knockout cell lysate controls to verify the expected 12 kDa band.
• For IF/ICC, standard fixation and permeabilization protocols can be employed, but optimization for your cell type is recommended.
• In IHC-P experiments, validate staining in relevant tissue samples and consider antigen retrieval conditions; SUMO1 expression levels may vary.
• Cross‑reactivity has been confirmed for human, mouse, and rat; reactivity with other species should be tested independently.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one‑stop solution for IVD raw material sourcing, catering to diagnostic manufacturers, laboratories, and research institutes. This anti‑SUMO1 polyclonal antibody supports investigations into ubiquitin‑like modifications, nuclear transport, and signal transduction. Our broad supply capabilities include validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare‑target raw materials to accelerate your assay development from concept to clinic.