Anti-SUMO1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P63165

Core Product Specifications and Parameters

Biological Background: SUMO1 Function and Localization

• Small ubiquitin-related modifier 1 (SUMO1) is a ubiquitin-like protein that becomes covalently attached to lysine residues of target proteins, a process known as SUMOylation.
• SUMOylation plays essential roles in nuclear transport, DNA replication and repair, mitosis, and signal transduction.
• SUMO1 can be conjugated as a monomer or form lysine-linked polymers; polymeric SUMO1 chains may be tagged with ubiquitin for proteasomal degradation.
• The protein localizes to multiple subcellular compartments, including the nucleus (nuclear membrane, nuclear speckles, PML bodies), cytoplasm, and cell membrane.
• SUMO1 modification of the voltage-gated potassium channel KCNB1 modulates its gating characteristics, linking SUMOylation to neuronal excitability. Related references: PMID:19223394
• SUMO1 is also covalently attached to ZFHX3, a transcription factor implicated in palate development and gene regulation. Related references: PMID:24651376
• Post-translational modifications of SUMO1 itself include acetylation and phosphorylation, which may regulate its function.
• It is involved in host-virus interactions and chromosomal rearrangements, highlighting its broad biological significance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human SUMO1 protein (amino acids 1-101), which may confer recognition of native SUMO1 and SUMOylated conjugates. Validate specificity by comparing wild-type and SUMO1 knockdown/knockout samples.
• For western blotting (WB), a major band at ~12 kDa corresponding to free SUMO1 is expected. Higher molecular weight bands may represent SUMO1-conjugated substrates; consider using a SUMOylation-specific inhibitor (e.g., ginkgolic acid) to confirm.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval and permeabilization may be required for optimal detection of nuclear and membrane-associated SUMO1 signals. Titrate antibody concentrations for specific staining in relevant tissue or cell systems.
• This polyclonal antibody has been validated for ELISA; when developing sandwich immunoassays, pair with a suitable capture/detection antibody after thorough cross-reactivity testing.
• The antibody cross-reacts with mouse and rat SUMO1 due to high sequence conservation. Confirm reactivity in your model system using appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with one-stop access to IVD raw materials, including validated polyclonal and monoclonal antibodies like this SUMO1 antibody. We offer optimized antibody pairs, bulk ancillary reagents, and sourcing for rare targets in the ubiquitin/SUMOylation pathway. From concept to clinic, CamelBio delivers reliable supply solutions and technical consultation to accelerate your assay development.