Anti-SUMF2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q8NBJ7

Core Product Specifications and Parameters

Biological Background: SUMF2 Function and Localization

• SUMF2, also known as pFGE (paralog of formylglycine-generating enzyme) or sulfatase-modifying factor 2, is an inactive paralog of SUMF1, the enzyme responsible for converting inactive sulfatases to their active form.
• It lacks formylglycine generating activity and cannot activate newly synthesized sulfatases; instead, it inhibits sulfatase activation by SUMF1.
• The protein localizes to the lumen of the endoplasmic reticulum, where post-translational modification of sulfatases occurs.
• SUMF2 is widely expressed in human tissues, with highest levels detected in kidney, liver, and placenta.
• Key post-translational modifications include glycosylation and disulfide bond formation; a calcium-binding site is also predicted.
• A three-dimensional structure is available (UniProt keyword: 3D-structure), providing insights into its interaction with SUMF1 and sulfatases.

Experimental Guidance and Technical Tips

• The immunogen used (aa 45-320) corresponds to the mature SUMF2 protein, likely recognizing native conformations in WB and IF/ICC.
• For Western blot, a band around 34 kDa is expected; validate in lysates from tissues with high expression (e.g., kidney, liver, placenta).
• In IF/ICC, permeabilization is required to access the ER lumen; consider co-staining with an ER marker to confirm localization.
• Cross-reactivity with mouse and rat has been reported; however, sequence alignment of the immunogen region is advisable for other species.
• The antibody is also suitable for ELISA; optimal coating and detection conditions should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined one-stop sourcing experience for IVD raw materials, technical services, and consulting. This anti-SUMF2 polyclonal antibody supports research into sulfatase activation, lysosomal storage disorders, and endoplasmic reticulum biology. Beyond this product, CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist with the sourcing of rare-target raw materials to accelerate your assay development pipeline.