Anti-SULT2B1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O00204

Core Product Specifications and Parameters

Biological Background: SULT2B1 Function and Localization

• SULT2B1, a member of the cytosolic sulfotransferase family, is encoded by the SULT2B1 gene and exists in tissue-specific isoforms via alternative splicing.
• It catalyzes the transfer of a sulfonate group from 3'-phospho-5'-adenylyl sulfate (PAPS) to hydroxyl groups of substrates, primarily sulfating cholesterol and hydroxysteroids like pregnenolone and dehydroepiandrosterone (DHEA). Related references: PMID:12145317, PMID:16855051, PMID:21855633
• Plays a critical role in epidermal cholesterol metabolism and regulation of keratinocyte proliferation/differentiation, as evidenced by expression at the stratum granulosum-stratum corneum junction. Related references: PMID:28575648
• Localized to the cytoplasm, microsome, and nucleus, enabling sulfation activity in multiple cellular compartments.
• Expressed at high levels in placenta, prostate, and trachea, with lower levels in small intestine and lung. Related references: PMID:9799594
• Post-translationally modified by phosphorylation, and genetic variations are linked to the skin disorder ichthyosis.

Experimental Guidance and Technical Tips

• Based on the full-length immunogen, this rabbit polyclonal antibody is expected to recognize multiple epitopes, potentially enabling detection of both native and denatured SULT2B1 protein in applications such as WB and IF/ICC.
• For WB, a band approximately 41 kDa is observed; loading controls should be included, and validation in human, mouse, or rat tissue lysates (e.g., placenta or prostate) is recommended.
• For IF/ICC, fixation and permeabilization conditions should be optimized, and nuclear or cytoplasmic staining may be observed consistent with SULT2B1 localization.
• ELISA applications may require titration of the capture/detection antibody concentrations; consider pairing with a validated SULT2B1 standard protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a seamless sourcing experience for IVD raw materials like this anti-SULT2B1 polyclonal antibody, supporting studies in steroid metabolism and dermatological disease. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate assay development. For specialized needs, we also source rare-target raw materials, ensuring a complete supply chain from concept to clinic.