Anti-SULT2A1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q06520

Core Product Specifications and Parameters

Biological Background: SULT2A1 Function and Localization

• SULT2A1 is a sulfotransferase that uses 3'-phospho-5'-adenylyl sulfate (PAPS) as a sulfonate donor to catalyze the sulfonation of a wide range of steroids and sterols, including pregnenolone, androsterone, dehydroepiandrosterone (DHEA), bile acids, cholesterol, and many xenobiotics with alcohol or phenol groups (PubMed:14573603, PubMed:18042734, PubMed:19589875). Related references: PMID:14573603 PMID:18042734 PMID:19589875
• Plays an important role in maintaining steroid and lipid homeostasis, as sulfonation increases water solubility and renal excretion of steroids (PubMed:14573603, PubMed:19589875, PubMed:21187059). Related references: PMID:14573603 PMID:19589875 PMID:21187059
• Key enzyme in bile acid metabolism, mediating the formation of 3-sulfated bile acids, which are major components of sulfate-conjugated bile acids in human urine (PubMed:2268288, PubMed:20102295). Related references: PMID:2268288 PMID:20102295
• Also catalyzes the metabolic activation of potent carcinogenic polycyclic arylmethanols via sulfonation (By similarity).
• Subcellular localization: cytoplasm.
• Tissue specificity: highly expressed in liver and adrenal gland, with lower levels in kidney; expression is higher in fetal adrenal than in fetal liver or kidney.
• Belongs to the sulfotransferase family; also known as hydroxysteroid sulfotransferase (HST), DHEA-ST, and bile salt sulfotransferase, with multiple aliases including ST2, STD, hSTa, ST2A1, ST2A3, and DHEA-ST8.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is approximately 34 kDa; consider using a positive control such as human liver or adrenal tissue lysate.
• For immunohistochemistry-paraffin sections (IHC-P), optimize antigen retrieval and validate staining in relevant tissues including liver and adrenal gland, where SULT2A1 expression is high.
• This antibody has been shown to cross-react with mouse and rat; validate in your sample system if using non-human models.
• ELISA applications are supported; ensure appropriate antigen coating and detection conditions are optimized for your assay format.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we provide a comprehensive one-stop sourcing bridge for IVD raw materials, serving diagnostic manufacturers and research laboratories worldwide. This SULT2A1 polyclonal antibody exemplifies our commitment to offering validated antibody pairs, optimized mono- and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—streamlining your steroid metabolism or liver function assay development from concept to clinic. Let us support your next project with reliable, high-quality raw materials and technical consulting.