Anti-SULT2A1 Rabbit Monoclonal Antibody for WB, IF-P, ELISA - Q06520

Core Product Specifications and Parameters

Biological Background: SULT2A1 Function and Localization

• SULT2A1 is a sulfotransferase that utilizes PAPS as a sulfonate donor to catalyze the sulfonation of steroids and bile acids (PubMed:14573603, PubMed:18042734, PubMed:19589875). Related references: PMID:14573603 PMID:18042734 PMID:19589875

• It mediates the sulfation of a broad range of compounds including pregnenolone, DHEA, bile acids, cholesterol, and xenobiotics, contributing to steroid and lipid homeostasis (PubMed:14573603, PubMed:19589875, PubMed:21187059). Related references: PMID:14573603 PMID:19589875 PMID:21187059

• Plays a critical role in bile acid metabolism by generating 3-sulfated bile acids (PubMed:2268288, PubMed:20102295). Related references: PMID:2268288 PMID:20102295

• Localizes to the cytoplasm.

• Expressed predominantly in liver, adrenal gland, and at lower levels in kidney; fetal expression is higher in adrenal.

• Synonyms include HST, ST2, STD, DHEA-ST, ST2A3, and hydroxysteroid sulfotransferase.

• Classified as a phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 180-285 of human SULT2A1 (Q06520), a region that may be conserved across species; consider sequence alignment to assess cross-reactivity with homologs from rat or other species.
• For Western blot, expect a band at ~34 kDa. Validate with appropriate controls, such as SULT2A1 knockout or siRNA-treated lysates.
• In immunofluorescence, SULT2A1 is cytoplasmic; ensure adequate permeabilization and include organelle markers for colocalization studies.
• For ELISA, optimize coating and blocking conditions; paired antibody sets may improve assay sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials for metabolism-related targets like SULT2A1, a critical enzyme in steroid and bile acid homeostasis. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline assay development. For rare targets or custom needs, our sourcing team can efficiently bridge supply gaps.