Anti-SULT1C4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O75897

Core Product Specifications and Parameters

Biological Background: SULT1C4 Function and Localization

• Sulfotransferase 1C4 (SULT1C4), also known as SULT1C2, is a member of the sulfotransferase 1 family that catalyzes the transfer of a sulfonate group from 3'-phospho-5'-adenylyl sulfate (PAPS) to phenolic compounds.
• It plays a key role in the metabolism of xenobiotics, including dietary flavonoids (phytoestrogens) and environmental estrogens like bisphenol A. These substrates are sulfated more efficiently than 17beta-estradiol. Related references: PMID:17425406 PMID:26948952 PMID:28222028
• SULT1C4 can also sulfate anthracycline drugs such as doxorubicin and its analog epirubicin, suggesting a role in modulating the efficacy and toxicity of these antitumor agents. Related references: PMID:26948952
• The enzyme is predominantly localized in the cytoplasm/cytosol, consistent with its function in Phase II detoxification.
• It is highly expressed in fetal lung and kidney, with lower expression detected in fetal heart, adult kidney, ovary, and spinal cord.
• Alternative splicing of the SULT1C4 gene generates multiple transcript variants, potentially expanding functional diversity.
• The predicted molecular weight of the canonical isoform is approximately 35.5 kDa, while this antibody detects a band at ~36 kDa in denaturing Western blots.

Experimental Guidance and Technical Tips

• For Western blotting, start with a standard dilution range and optimize based on sample type and expression level; a band near 36 kDa is expected.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative assays; consider performing a bridging study when switching lots.
• Cross-reactivity with mouse and rat SULT1C4 homologs makes this antibody suitable for studies in common rodent models.
• For immunofluorescence/immunocytochemistry, fixation and permeabilization conditions should be empirically determined; mild fixation (e.g., 4% PFA) is often effective for cytosolic proteins.
• ELISA protocols can be developed using either direct antigen coating or a capture format; validate the assay with a known positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Whether you need SULT1C4 antibodies for drug metabolism research or rare-target sourcing for custom assay development, our streamlined supply chain bridges the gap from concept to clinic. Contact us to fulfill your entire raw-material needs with a single reliable partner.