Anti-SULT1A2 Polyclonal Antibody for WB, ELISA - P50226

Core Product Specifications and Parameters

Biological Background: SULT1A2 Function and Localization

• SULT1A2 (Sulfotransferase 1A2) is a cytosolic enzyme that catalyzes the transfer of a sulfonate group from the universal donor 3'-phospho-5'-adenylyl sulfate (PAPS) to various endogenous and xenobiotic substrates.
• Also known as Aryl sulfotransferase 2, Phenol sulfotransferase 2, or P-PST 2 (phenol-sulfating phenol sulfotransferase 2), it plays a key role in the metabolism of catecholamines, phenolic drugs, and neurotransmitters.
• This enzyme mediates the sulfonation and bioactivation of the hypotensive agent minoxidil, and is involved in the metabolic activation of carcinogenic N-hydroxyarylamines to DNA-reactive products.
• SULT1A2 localizes predominantly to the cytoplasm.
• The canonical isoform has a calculated molecular weight of approximately 34 kDa (34310 Da) and is annotated with keywords including catecholamine metabolism, lipid metabolism, steroid metabolism, and transferase activity.

Experimental Guidance and Technical Tips

• The antibody was generated against a recombinant fragment spanning amino acids 201–295 of human SULT1A2, targeting the C-terminal region. This design may allow recognition of the full-length protein and orthologs in other species.
• For Western blot, expect a band near 34 kDa. Use appropriate positive controls (e.g., HepG2, liver lysates) and pre-stained molecular weight markers to verify specificity.
• For ELISA, the antibody can be considered for both direct antigen detection and capture configurations; optimal working concentrations should be determined experimentally.
• Since cross-reactivity with mouse and rat proteins is indicated, we recommend validating performance in your sample type of interest (tissue homogenates, cell lysates, serum/plasma) to ensure consistent reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated one-stop sourcing platform for IVD raw materials, offering this anti-SULT1A2 polyclonal antibody alongside a wide range of validated reagents for drug metabolism, toxicology, and cancer research. Our capabilities include bulk supply of monoclonal and polyclonal antibodies, antigen-grade proteins, and ancillary reagents, with the flexibility to source rare targets and develop custom solutions to accelerate your diagnostic assay development.