Anti-SULT1A1 Rabbit Polyclonal Antibody for WB and ELISA - P50225

Core Product Specifications and Parameters

Biological Background: SULT1A1 Function and Localization

• SULT1A1 is a cytosolic sulfotransferase that catalyzes the sulfate conjugation of phenolic compounds, hormones, and xenobiotics using PAPS as a donor. Related references: PMID:12471039, PMID:16221673, PMID:21723874
• Plays a key role in the metabolism of endogenous steroids and catecholamines, including the sulfonation of dopamine and thyroid hormones (T4, T3, rT3, 3,3'-T2), with a substrate preference of 3,3'-T2 > rT3 > T3 > T4. Related references: PMID:8093002, PMID:10199779
• Mediates the bioactivation of procarcinogens such as N-hydroxyarylamines, leading to DNA adduct formation and potential mutagenesis. Related references: PMID:7834621
• Involved in gut microbiota–host metabolic interactions by O-sulfonating the microbial metabolite 4-ethylphenol (4-EP); the product 4-EPS may impact brain myelination and limbic system connectivity. Related references: PMID:35165440
• Also known by multiple aliases including P-PST, ST1A3, and HAST1/HAST2, reflecting its phenol sulfotransferase and thermostable properties.
• Predominantly expressed in the liver, lung, adrenal gland, brain, platelets, and skin, consistent with its roles in detoxification and hormone regulation.
• Subject to alternative splicing and phosphorylation, with a molecular weight of approximately 34 kDa (calculated from sequence) and a crystal structure resolved.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human SULT1A1 protein (amino acids 1–295), suggesting the antibody may recognize multiple epitopes and could be effective for detecting both native and denatured forms in applications like Western blotting.
• For Western blot, anticipate a primary band at approximately 34 kDa in human, mouse, and rat tissue lysates; consider using reducing conditions and verifying expression in liver or lung samples where SULT1A1 is abundant.
• In ELISA, the antibody may be used as a capture or detection reagent; appropriate pairing with a matched antibody and validation against recombinant SULT1A1 protein is recommended.
• As a rabbit polyclonal antibody, lot-specific affinity and background may vary; optimize dilution factors and include appropriate controls (e.g., SULT1A1-overexpressing cell lysates or siRNA knockdown) to confirm specificity.

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