Anti-SULF1 Rabbit Polyclonal Antibody for WB, ELISA - Q8IWU6

Core Product Specifications and Parameters

Biological Background: SULF1 Function and Localization

• SULF1 (Extracellular sulfatase Sulf-1) is also known as arylsulfatase or N-acetylglucosamine-6-sulfatase, encoded by the SULF1 gene. It is a member of the sulfatase family and functions as a heparin-degrading endosulfatase.
• Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity, removing sulfate from the C-6 position of glucosamine within specific subregions of intact heparin (PubMed:12368295, PubMed:12686563).
  Related references: PMID:12368295 PMID:12686563
• Diminishes heparan sulfate proteoglycan (HSPG) sulfation, thereby inhibiting signaling by heparin-dependent growth factors, reducing cell proliferation, and facilitating apoptosis in response to exogenous stimulation (PubMed:12686563).
  Related references: PMID:12686563
• Localizes to the endoplasmic reticulum, Golgi apparatus, Golgi stack, and is also found on the cell surface and secreted into the extracellular space.
• Shows highest expression in testis, stomach, skeletal muscle, lung, kidney, pancreas, small intestine, and colon. Down-regulation is observed in ovarian carcinoma, breast, pancreatic, renal, and hepatocellular carcinoma cell lines, indicating a potential tumor suppressor role.
• Key biological processes include apoptosis, calcium binding, and metal-binding; the protein is glycosylated and contains a signal peptide for secretion.
• Molecular weight of the full-length protein is approximately 101 kDa, consistent with the predicted 101027 Da from sequence.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 480–610 within the C-terminal region of human SULF1, which may provide specific detection of the target protein.
• For Western blot analysis, the predicted molecular weight is approximately 101 kDa; a band near this size is expected. Consider using appropriate positive controls, such as lysates from tissues with high SULF1 expression (e.g., testis, stomach).
• This antibody is suitable for ELISA applications; we recommend performing a checkerboard titration to determine optimal coating and detection antibody concentrations.
• Cross-reactivity is observed with rat SULF1, making it suitable for studies in rat models; validate in your specific sample system before routine use.
• The product is shipped on ice bags to maintain stability; store at the recommended temperature upon arrival.

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