Anti-SUGP2 Polyclonal Antibody for WB, ELISA - Q8IX01

Core Product Specifications and Parameters

Biological Background: SUGP2 Function and Localization

• SUGP2 (SURP and G-patch domain-containing protein 2), also known as SFRS14 or SRFS14, is a splicing factor containing SURP and G-patch domains.
• It is implicated in mRNA splicing, potentially through its domain-mediated RNA and protein interactions.
• SUGP2 localizes to the nucleus, consistent with its role in pre-mRNA processing.
• Expression is detected in adult testis, fetal brain, and fetal kidney, suggesting tissue-specific splicing regulation.
• The protein undergoes alternative splicing and is subject to post-translational modifications including phosphorylation and ubiquitination (isopeptide bond).
• SUGP2 belongs to a family of SURP and G-patch domain-containing proteins involved in splicing regulation.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is ~120 kDa; use an appropriate gel percentage for high molecular weight proteins and consider nuclear extracts to enhance detection.
• The immunogen (amino acids 500–700) may yield reactivity against multiple epitopes; validate specificity in relevant cell lines or tissues.
• In ELISA, the antibody can be used as a capture or detection reagent after titration and buffer optimization.
• Cross-reactivity is confirmed for human and mouse; verify performance in other species if needed.
• Tissue expression in testis, fetal brain, and kidney can serve as positive controls; include negative controls (e.g., knockdown or knockout) where possible.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to IVD raw materials like this SUGP2 antibody, supporting mRNA splicing-related assay development. Our portfolio includes validated antibody pairs, recombinant proteins, and ancillary reagents for immunodiagnostics. We also offer technical consulting to accelerate your project from concept to clinic.