Anti-SUGCT Polyclonal Antibody for WB, ELISA - Q9HAC7

Core Product Specifications and Parameters

Biological Background: SUGCT Function and Localization

• SUGCT (Succinyl-CoA:glutarate CoA-transferase) is a mitochondrial enzyme encoded by the SUGCT gene, also known as Dermal papilla-derived protein 13 (DERP13), with a molecular weight of ~48 kDa.
• It catalyzes the reversible transfer of CoA from dicarboxyl-CoAs (preferring succinyl-CoA and glutaryl-CoA) to dicarboxylates including glutarate, succinate, adipate, itaconate, and 3-hydroxy-3-methylglutarate, playing a key role in metabolite recycling (PubMed:23893049, PubMed:34492704, PubMed:38915184). Related references: PMID:23893049, PMID:34492704, PMID:38915184
• SUGCT acts in the lysine and tryptophan degradation pathway, catalyzing succinyl-CoA-dependent reesterification of free glutarate to glutaryl-CoA to prevent renal loss (PubMed:18926513, PubMed:23893049, PubMed:38915184). Related references: PMID:18926513, PMID:23893049, PMID:38915184
• During inflammation, SUGCT may convert macrophage-derived itaconate to itaconyl-CoA in erythroid precursors, thereby negatively regulating the TCA cycle, heme synthesis, and stress erythropoiesis (PubMed:34492704). Related references: PMID:34492704
• Immunofluorescence and electron microscopy studies localize SUGCT predominantly to mitochondria.
• Tissue expression analysis shows high expression in kidney, with moderate levels in liver, skeletal muscle, and pancreas, and low/absent expression elsewhere.
• UniProt keywords highlight the protein as a transferase with a transit peptide, acetylation sites, and involvement in glutaric aciduria pathology; alternative splicing and disease variants are documented.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal 250 amino acids of human SUGCT, potentially enabling detection of full-length and processed forms; consider the transit peptide cleavage site when assessing band sizes in Western blotting.
• For Western blotting, the predicted molecular weight is ~48 kDa (precursor) or ~45 kDa (mature); validate in lysates from kidney or liver tissues where expression is high.
• For ELISA, optimize coating concentrations and validate in the relevant sample matrix; cross-reactivity with mouse and rat orthologs should be confirmed by sequence comparison.

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