Anti-SUFU Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9UMX1

Core Product Specifications and Parameters

Biological Background: SUFU Function and Localization

• SUFU (Suppressor of Fused Homolog), also known as SUFUH, SUFUXL, PRO1280, and JBTS32, is a key component of the Hedgehog signaling pathway. Multiple isoforms are produced by alternative splicing.
• Core function: acts as a negative regulator in the Hedgehog/Smoothened pathway by sequestering GLI transcription factors (GLI1, GLI2, GLI3) in the cytoplasm, preventing their nuclear translocation and target gene activation. Related references: PMID:10559945, PMID:10564661, PMID:10806483
• In the absence of Hedgehog signaling, SUFU forms a complex with full-length GLI3 and recruits it to cilia, promoting the processing of GLI3 into its transcriptional repressor form (GLI3R) by facilitating GSK3B recruitment. Related references: PMID:24311597, PMID:28965847
• Upon Smoothened activation, GLI transcription factors dissociate from SUFU, translocate to the nucleus, and induce expression of Hedgehog target genes. Related references: PMID:24311597, PMID:28965847, PMID:35831023
• Subcellular localization is dynamic: SUFU shuttles between the cytoplasm, nucleus, and primary cilium, reflecting its regulatory roles in signaling and ciliary trafficking.
• Ubiquitously expressed in adult tissues; isoform 1 is detected in fetal brain, lung, kidney, and testis, while isoform 2 is predominantly expressed in fetal testis and at lower levels in other fetal tissues.
• Classified as a tumor suppressor protein; post-translational modifications include phosphorylation, acetylation, and ubiquitination. Mutations are associated with ciliopathies such as Joubert syndrome 32 (JBTS32).
• May also function as a negative regulator of beta-catenin signaling, contributing to developmental processes and epidermal differentiation.

Experimental Guidance and Technical Tips

• Based on the immunogen design (C-terminal region, amino acids 325–484), the antibody is expected to recognize both major isoforms of SUFU. For Western blot, a predominant band around 54 kDa should be observed; minor bands representing isoforms or post-translationally modified forms may also be detected.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider antigen retrieval conditions and appropriate fixation methods. Since SUFU localizes to cytoplasm, nucleus, and cilia, ensure your imaging protocol can resolve these compartments.
• The antibody has been validated on human, mouse, and rat samples. For ELISA applications, use recombinant SUFU protein or cell lysates as coating antigens, and confirm specificity with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD development by offering a comprehensive range of high-quality raw materials and technical support. This anti-SUFU polyclonal antibody is well-suited for researchers studying Hedgehog signaling and for diagnostic assay manufacturers targeting related pathways. Beyond this product, CamelBio provides validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing services for rare targets, helping you move efficiently from concept to clinic.