Anti-SUCLG2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q96I99

Core Product Specifications and Parameters

Biological Background: SUCLG2 Function and Localization

• SUCLG2 encodes the beta subunit of the GTP-specific succinyl-CoA ligase (succinyl-CoA synthetase), an enzyme of the mitochondrial tricarboxylic acid (TCA) cycle that catalyzes the only substrate-level phosphorylation step, converting succinyl-CoA to succinate with the concomitant generation of GTP. Related references: PMID:40108300
• The enzyme also functions as an itaconyl-CoA and malyl-CoA ligase, highlighting broader substrate versatility in mitochondrial metabolism. Related references: PMID:40108300
• The beta subunit determines nucleotide specificity and binds the substrate succinate, while the alpha subunit harbors the coenzyme A and phosphate binding sites.
• As indicated by UniProt keywords, SUCLG2 undergoes alternative splicing, acetylation, and phosphorylation, and requires magnesium as a metal cofactor for activity.
• SUCLG2 is localized exclusively to the mitochondrion, consistent with its essential role in energy metabolism.
• Predominantly expressed in liver, kidney, heart, spleen, and skeletal muscle, with lower levels detected in intestine, colon, lung, brain, prostate, testis, and ovary, reflecting its tissue-specific metabolic functions.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 50–300 of human SUCLG2, a region conserved across human, mouse, and rat, supporting the antibody's cross-reactivity for multi-species studies.
• For western blot, the predicted band matches the 47 kDa protein weight; account for possible size shifts due to post-translational modifications such as acetylation or phosphorylation.
• In immunohistochemistry (IHC-P) and immunocytochemistry (IF/ICC), validate staining in tissues or cell lines with known high SUCLG2 expression, such as liver or kidney sections.
• For ELISA applications, the recombinant immunogen fragment can be employed as a positive control to assess antibody binding and assay sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner for metabolism and mitochondrial research reagents. In addition to this anti-SUCLG2 polyclonal antibody, we offer validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to streamline your diagnostic assay development. Our supply network covers rare targets and custom sourcing, supporting every stage from concept to clinic.