Anti-SUCLG1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P53597

Core Product Specifications and Parameters

Biological Background: SUCLG1 Function and Localization

• SUCLG1 encodes the alpha subunit of succinyl-CoA synthetase (SCS), also known as galactose activation protein (GALPHA) and associated with mitochondrial DNA depletion syndrome 9 (MTDPS9) upon mutation.
• SCS functions in the TCA cycle, coupling succinyl-CoA hydrolysis to ATP or GTP synthesis, representing the only substrate-level phosphorylation step (PubMed:34492704, PubMed:40108300). Related references: PMID:34492704, PMID:40108300
• The alpha subunit binds coenzyme A and phosphate, while beta subunits determine nucleotide specificity for ATP or GTP (By similarity).
• SUCLG1 also possesses itaconyl-CoA and malyl-CoA synthetase activity, highlighting metabolic flexibility (PubMed:40108300). Related references: PMID:40108300
• It is a mitochondrial protein, essential for cellular energy metabolism.
• Post-translational modifications, including acetylation, and disease-associated variants underline its role in primary mitochondrial disorders.
• With a predicted molecular weight of ~36 kDa, SUCLG1 is a conserved component of the ligase family.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 41–346, nearly the full length, suggesting the antibody may recognize the native protein in multiple assays.
• For Western blot, an ~36 kDa band is expected; consider using mitochondrial-enriched lysates to enhance signal.
• In immunocytochemistry/immunofluorescence, anticipate a mitochondrial staining pattern; co-staining with organelle markers is recommended.
• ELISA performance can be optimized with recombinant SUCLG1; validate in the relevant sample matrix and species.
• Cross-reactivity is reported for human, mouse, and rat; verify in other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD development with comprehensive raw-material solutions. This SUCLG1 antibody supports research in mitochondrial metabolism, TCA cycle disorders, and energy homeostasis. Beyond this product, we offer validated antibody pairs, recombinant proteins, and bulk ancillary reagents to accelerate your assay development and manufacturing pipeline.