Anti-SUCLA2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9P2R7

Core Product Specifications and Parameters

Biological Background: SUCLA2 Function and Localization

• SUCLA2 encodes the beta subunit of ATP-specific succinyl-CoA synthetase (A-SCS), a mitochondrial enzyme that catalyzes the conversion of succinyl-CoA to succinate coupled with ATP synthesis, the only substrate-level phosphorylation step in the TCA cycle. Related references: PMID:15877282, PMID:34492704, PMID:40108300
• The enzyme also functions as an ATP-specific itaconyl-CoA and malyl-CoA synthetase, expanding its metabolic role beyond the classic TCA cycle. Related references: PMID:40108300
• The beta subunit determines nucleotide specificity (ATP vs GTP) and contains the succinate-binding site, while the alpha subunit provides binding sites for coenzyme A and phosphate.
• Localized to the mitochondrial matrix, consistent with its central role in energy metabolism.
• Widely expressed in human tissues, with the exception of liver and lung, indicating tissue-specific metabolic demands.
• Predicted molecular weight of approximately 50 kDa, with post-translational modifications such as acetylation and phosphorylation potentially regulating function.
• Alternative splicing produces multiple transcript variants, which may contribute to functional diversity.
• Genetic variants in SUCLA2 are linked to mitochondrial DNA depletion syndrome and other metabolic disorders, underscoring its clinical relevance.

Experimental Guidance and Technical Tips

• The antibody was raised against an N-terminal fragment (aa 1-180) of human SUCLA2; this region is conserved across species, consistent with its cross-reactivity to mouse and rat, but we recommend verification in your specific sample system.
• In Western blot, a predominant band at approximately 50 kDa should be detected; including positive controls (e.g., HEK293, HeLa lysates) can aid interpretation.
• For immunocytochemistry/immunofluorescence, anticipate mitochondrial staining; co-staining with mitochondrial markers is advisable for confirmation.
• ELISA applications may require optimization of coating antigen and antibody concentration; consider using the recombinant immunogen as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop B2B sourcing partner for high-quality IVD raw materials, supporting diagnostic developers from concept to clinic. This anti-SUCLA2 polyclonal antibody is ideal for research into mitochondrial metabolism and related disorders. Beyond catalog antibodies, we offer custom antibody development, bulk reagent supply, and rare target sourcing to meet your project's unique needs.