Anti-SUB1 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P53999

Core Product Specifications and Parameters

Biological Background: SUB1 Function and Localization

• SUB1 encodes activated RNA polymerase II transcriptional coactivator p15, also known as positive cofactor 4 (PC4), p14, or SUB1 homolog.
• As a general coactivator, p15/PC4 cooperates with TATA-box binding protein-associated factors (TAFs) and mediates functional interactions between upstream activators and the general transcriptional machinery, possibly stabilizing the multiprotein transcription complex.
• The protein binds single-stranded DNA and, in vitro, non-specifically to double-stranded DNA (dsDNA), suggesting a role in transcription initiation or chromatin engagement.
• SUB1 is localized to the nucleus, consistent with its function in transcriptional regulation.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation (isopeptide bond), which may modulate its activity or interactions.
• Structural studies (3D-structure keyword) have provided insights into its DNA-binding and protein interaction surfaces.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-127 of human SUB1, covering the full-length protein; this design may detect both endogenous and recombinant protein across multiple applications.
• For western blot, anticipate a band near 14 kDa under denaturing and reducing conditions; validate specificity using appropriate controls and blocking agents.
• Immunofluorescence/ICC should reveal nuclear staining; include DAPI co-stain and no‑primary negative controls to confirm localization.
• For immunoprecipitation, optimize antibody‑to‑lysate ratio and consider cross‑linking if target DNA interaction may mask epitopes.
• ELISA detection may require testing direct vs. sandwich format with recombinant protein standards for quantitation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material provider, offering validated antibody pairs, bulk ancillary reagents, and customized sourcing for molecules involved in transcription and epigenetics. This SUB1 rabbit polyclonal antibody is part of our portfolio designed to support diagnostic developers and research laboratories from feasibility to market. Our team ensures efficient procurement and technical consultation to help you accelerate assay development for transcriptional coactivators and related targets.