Anti-STXBP4 Polyclonal Antibody for WB, IHC-P, ELISA - Q6ZWJ1

Core Product Specifications and Parameters

Biological Background: STXBP4 Function and Localization

• STXBP4 (Syntaxin-binding protein 4) encodes a protein also known as Synip or STX4-interacting protein, with alternative splicing events indicated by UniProt.
• The protein functions in the translocation of transport vesicles from the cytoplasm to the plasma membrane, a critical step in intracellular trafficking.
• It negatively regulates SLC2A4/GLUT4 glucose transporter translocation by binding STX4A and preventing the STX4A-VAMP2 SNARE complex formation, thereby retaining GLUT4 in intracellular vesicles.
• Insulin stimulation disrupts the STXBP4-STX4A interaction, leading to increased SLC2A4 levels at the plasma membrane and enhanced glucose uptake.
• STXBP4 may also play a role in the regulation of insulin release from pancreatic beta cells upon glucose stimulation.
• The protein localizes to the cytoplasm and contains structural features such as coiled-coil domains and repeat sequences.
• It is classified as a phosphoprotein, suggesting regulation by phosphorylation, and has a molecular weight of approximately 62 kDa.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 344–553, a region likely conserved between human and mouse, supporting the reported cross-reactivity.
• For Western blot, consider using whole-cell lysates from insulin-responsive tissues (e.g., adipose, muscle) or cell lines; expect a band near 62 kDa.
• For IHC-P, validate in tissue sections known to express STXBP4, such as pancreas or adipose tissue; optimization of antigen retrieval may be needed.
• ELISA performance should be evaluated using recombinant STXBP4 protein or lysates, with proper controls for specificity.
• Since STXBP4 function is modulated by insulin, consider experimental designs involving insulin stimulation or glucose treatment to study its dynamic interactions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and laboratories with reliable antibodies and ancillary reagents. This anti-STXBP4 polyclonal antibody addresses the growing need for robust reagents in metabolic disease and insulin signaling research. Beyond this product, CamelBio offers validated antibody pairs, bulk monoclonal/polyclonal antibodies, and custom sourcing for rare targets, streamlining your entire product development pipeline.