Anti-STXBP3 Polyclonal Antibody for WB and ELISA - O00186

Core Product Specifications and Parameters

Biological Background: Syntaxin-binding Protein 3 Function and Localization

• Syntaxin-binding protein 3 (STXBP3, also known as MUNC18C, PSP, Unc-18C) is a member of the Sec1/Munc18 protein family that regulates SNARE-mediated vesicle docking and fusion.
• STXBP3 together with STX4 and VAMP2 may play a role in insulin-dependent GLUT4 trafficking—mediating movement and docking/fusion of GLUT4-containing vesicles at the adipocyte cell surface.
• Expressed primarily in megakaryocytes and platelets; participates in platelet exocytosis.
• Subcellularly localizes to the cytoplasm and cytosol, and can associate with the cell membrane upon stimulation.
• Key functional domains and post-translational modifications, including phosphorylation, modulate its interaction with syntaxin partners and vesicle fusion.
• Relevant keywords: Cell membrane, Cytoplasm, Membrane, Phosphoprotein, Protein transport, Transport.

Experimental Guidance and Technical Tips

• The immunogen spans residues 343–592, a region that may be conserved across species, consistent with the predicted cross-reactivity to mouse and rat STXBP3.
• For western blotting, a band near 68 kDa is expected; consider using positive controls from human, mouse, or rat cell lysates, particularly adipocytes or platelet-rich samples.
• In ELISA applications, the antibody may detect native or recombinant STXBP3; validate in the relevant sample matrix and consider blocking peptide competition to confirm specificity.
• Non-reducing conditions or optimized sample preparation may improve detection depending on the target’s phosphorylation state and subcellular localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a comprehensive, one-stop sourcing solution for IVD raw materials. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist with rare target procurement. For STXBP3-related vesicle trafficking and metabolic disease research, our portfolio includes ready-to-ship antibodies and flexible customization options to support your assay development and production needs.