Anti-STXBP2 Rabbit Polyclonal Antibody for WB and ELISA - Q15833

Core Product Specifications and Parameters

Biological Background: STXBP2 Function and Localization

• STXBP2 (Syntaxin-binding protein 2), also known as Munc18-2, UNC18B, or UNC18-2, is a member of the Sec1/Munc18 (SM) protein family critical for SNARE-mediated membrane fusion.
• It functions in intracellular vesicle trafficking by binding to syntaxins and promoting the assembly of functional SNARE complexes, particularly involving STX11, SNAP23, and VAMP8 at the plasma membrane.
• In natural killer (NK) cells, STXBP2 regulates cytotoxic granule exocytosis, essential for immune defense against infected or transformed cells.
• The protein localizes to the cell membrane and the membrane of cytolytic granules, consistent with its role in granule docking and fusion.
• STXBP2 is expressed in multiple tissues, including placenta, lung, liver, kidney, pancreas, and peripheral blood lymphocytes.
• Keywords associated with the protein include alternative splicing, disease variant, exocytosis, lysosome, and protein transport, highlighting its regulatory roles and clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–280) of human STXBP2; consider verifying reactivity against full-length recombinant protein or endogenous protein in relevant cell lysates.
• For Western blot (WB), a band at approximately 66 kDa is anticipated in human, mouse, and rat samples. Optimize loading controls and antibody dilution according to the specific experimental system.
• For ELISA, ensure appropriate coating antigen and detection conditions; recombinant STXBP2 may serve as a reference.
• Given STXBP2 involvement in exocytosis and disease-linked variants, confirm isoform detection if studying disease models, and validate specificity using siRNA knockdown or STXBP2-deficient cell lines where possible.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including this anti-STXBP2 rabbit polyclonal antibody. For projects exploring SNARE-mediated membrane fusion, cytotoxic granule exocytosis, or immune cell function, CamelBio offers validated antibody pairs, bulk ancillary reagents, and tailored sourcing support to streamline your assay development from concept to clinic.