Anti-STXBP1 Polyclonal Antibody for IF/ICC, IF-P, ELISA - P61764

Core Product Specifications and Parameters

Biological Background: STXBP1 Function and Localization

• STXBP1 (Syntaxin-binding protein 1), also known as MUNC18-1, N-Sec1, or p67, is a highly conserved protein predominantly expressed in the brain and spinal cord, with particular enrichment in axons.
• It plays a critical role in the regulation of synaptic vesicle docking and fusion by interacting with GTP-binding proteins, thereby ensuring efficient neurotransmission.
• STXBP1 binds to syntaxins 1, 2, and 3 (but not syntaxin 4) in a 1:1 ratio, facilitating the assembly of the SNARE complex at synaptic membranes, which is essential for neurotransmitter release.
• Subcellular localization includes the cytoplasm, cytosol, and membrane compartments, consistent with its role in vesicle trafficking and fusion.
• Post-translational modifications such as phosphorylation (phosphoprotein) and alternative splicing contribute to its functional diversity.
• Mutations in STXBP1 are linked to neurological disorders, including early infantile epileptic encephalopathy (DEE4) and intellectual disability, underscoring its importance in normal brain function.

Experimental Guidance and Technical Tips

• For immunofluorescence applications (IF/ICC and IF-P), consider optimizing permeabilization conditions to ensure antibody access to the intracellular target.
• In ELISA assays, validate the antibody against the specific recombinant protein immunogen (amino acids 295-594) to confirm reactivity and minimize background.
• Given that this is a polyclonal antibody, it is advisable to evaluate lot-to-lot consistency when establishing critical assays.
• The antibody’s cross-reactivity with rat STXBP1 enables validation in rodent brain tissue, a common model for synaptic studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw-material sourcing, offering a comprehensive portfolio that includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing. This STXBP1 polyclonal antibody is part of our extensive neuroscience research collection, supporting diagnostic manufacturers and laboratories in developing assays for synaptic function and neurological disorders. Let CamelBio bridge the gap from concept to clinic with reliable, research-grade materials and expert support.