Anti-STX8 Polyclonal Antibody for WB, ELISA - Q9UNK0

Core Product Specifications and Parameters

Biological Background: STX8 Function and Localization

• STX8 (Syntaxin-8) is a member of the syntaxin family of SNARE proteins involved in vesicle trafficking.
• It functions in the early secretory pathway, potentially mediating retrograde transport from cis-Golgi membranes to the endoplasmic reticulum (ER).
• The protein is predicted to contain a coiled-coil domain and a transmembrane helix, consistent with its membrane localization.
• Subcellularly, STX8 is associated with membrane compartments.
• Expression analysis shows high levels in the heart, with additional expression in brain, kidney, liver, lung, placenta, skeletal muscle, spleen, and pancreas.
• Post-translational modifications include phosphorylation (based on proteomics identification) and potential ubiquitin conjugation (ubl conjugation keyword).
• Its molecular weight is approximately 27 kDa (calculated ~26.9 kDa), and no splice isoforms are reported in UniProt.

Experimental Guidance and Technical Tips

• For Western blot, validate the antibody in the relevant sample system (human, mouse, or rat) to confirm specificity and optimal loading.
• The immunogen spans the N-terminal cytoplasmic domain (amino acids 1-215) of STX8, which is expected to recognize full-length protein; nonetheless, consider performing a blocking peptide or knockdown control to verify band identity.
• Given the predicted molecular weight of ~27 kDa, adjust gel percentage and transfer conditions to achieve clear separation.
• For ELISA applications, standard indirect ELISA protocols can be used; however, titrate the antibody and validate the assay dynamic range with recombinant STX8 protein or lysate standards.
• Storage and handling: Shipped on ice bag, aliquoting upon arrival and storing at -20°C or -80°C is recommended to avoid freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop provider of IVD raw materials, supporting diagnostic developers and researchers with high-quality antibodies and reagents. This anti-STX8 polyclonal antibody complements our portfolio for intracellular trafficking and membrane biology studies, and we can further assist with sourcing validated antibody pairs, optimized monoclonal or polyclonal antibodies, and bulk ancillary reagents for your assay development. Contact our team for custom protein expression or rare target sourcing needs.