Anti-STX6 Rabbit Polyclonal Antibody for WB, ELISA - O43752

Core Product Specifications and Parameters

Biological Background: Syntaxin-6 Function and Localization

• Syntaxin-6 is a SNARE protein encoded by the STX6 gene, belonging to the syntaxin family of membrane fusion regulators.
• It functions in vesicle trafficking, promoting movement of transport vesicles to target membranes. It targets endosomes to the trans-Golgi network, mediating retrograde trafficking.
• Together with SNARE STX12, it facilitates movement of vesicles from endosomes to the cell membrane, playing a role in the endocytic recycling pathway.
• Subcellularly, Syntaxin-6 localizes to the Golgi apparatus membrane, trans-Golgi network membrane, and recycling endosome membrane, consistent with its trafficking functions.
• Post-translational modifications include acetylation and phosphorylation, as indicated by UniProt keywords, which may regulate its activity or interactions.
• Alternative splicing of STX6 produces multiple isoforms, potentially diversifying its functional roles.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1‑234 of human STX6, a substantial N‑terminal region that may recognize multiple isoforms or be robust to certain modifications.
• For Western blot, consider using a standard protocol with an appropriate dilution based on the ~29 kDa target. Validate in human and mouse samples given the reported cross‑reactivity.
• For ELISA, the antibody’s performance may be optimized using recombinant STX6 protein as a standard; titrate the antibody in the relevant sample matrix.
• Store the antibody as recommended; it is shipped in an ice bag to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a reliable one‑stop sourcing partner for IVD manufacturers and research labs, offering a comprehensive range of validated antibodies, antigens, and ancillary reagents. This anti‑STX6 polyclonal antibody complements our portfolio for studying Golgi‑to‑endosome trafficking and endocytic recycling, with support available for bulk orders, custom antibody development, and technical consulting to accelerate your diagnostic or research workflows.