Anti-STX2 Rabbit Polyclonal Antibody for WB, ELISA - P32856

Core Product Specifications and Parameters

Biological Background: Syntaxin-2 Function and Localization

• Syntaxin-2 (STX2), also known as Epimorphin, belongs to the syntaxin family of SNARE proteins and is encoded by the STX2 gene in humans. Multiple splice variants include STX2A, STX2B, and STX2C (UniProt P32856).
• The protein is essential for epithelial morphogenesis, facilitating tissue organization and repair processes.
• STX2 may mediate calcium-dependent regulation of exocytosis, particularly in the acrosomal reaction of sperm.
• It localizes to the membrane and possesses a coiled-coil domain and a transmembrane helix, consistent with its role in vesicle fusion.
• Post-translational modifications and alternative splicing generate diverse isoforms, potentially modulating its function in different cell types (Keywords: Alternative splicing, Coiled coil, Transmembrane, Transmembrane helix, Membrane).
• The canonical isoform has a molecular weight of approximately 33 kDa (33341 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–264) of human STX2. Ensure that detection reagents (e.g., secondary antibodies) do not cross-react with this region in assays.
• For Western blot (WB), consider using epithelial cell lines or tissues known to express STX2, such as skin, intestine, or lung.
• Expect a band around 33 kDa; however, alternative splicing may produce isoforms of varying sizes, so validate in the relevant sample system.
• Cross-reactivity with mouse and rat has been reported, making this antibody suitable for translational studies using rodent models.
• In ELISA, optimize coating buffer and antibody dilutions to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a comprehensive one-stop platform for IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies like this anti-STX2 pAb. Our supply chain supports rare-target sourcing, bulk ancillary reagents, and custom technical services to accelerate your epithelial morphogenesis or exocytosis research projects from concept to clinic. Contact us for tailored procurement solutions and expert consultation.