Anti-STX17 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P56962

Core Product Specifications and Parameters

Biological Background: STX17 Function and Localization

• STX17 (Syntaxin-17) is a member of the syntaxin family of SNARE proteins, which play essential roles in intracellular membrane fusion.
• Acts as a key SNARE on autophagosomes, directly mediating the fusion of autophagosomes with lysosomes to form autolysosomes, a critical step in autophagy. Related references: PMID:23217709, PMID:25686604, PMID:28306502
• May also function in the early secretory pathway, possibly contributing to the structural maintenance of the ER–Golgi intermediate compartment (ERGIC) and Golgi apparatus, and/or regulating trafficking between the endoplasmic reticulum, ERGIC, and Golgi. Related references: PMID:21545355
• Exhibits a complex subcellular distribution, localizing to membranes of the smooth endoplasmic reticulum, ERGIC, autophagosomes, COPII-coated vesicles, mitochondria, and autolysosomes, as well as in the cytosol.
• The human STX17 protein has a predicted molecular weight of approximately 33 kDa.
• Post-translational modifications including acetylation and phosphorylation have been identified, which may regulate its function or localization.

Experimental Guidance and Technical Tips

• The immunogen, corresponding to the N-terminal region (amino acids 1–130), is ideal for detecting total STX17 across applications.
• For Western blot, anticipate a band at ~33 kDa; optimize blocking and antibody dilution for your specific sample type and consider using a low-background detection system.
• In immunofluorescence/ICC, expect punctate staining patterns indicative of autophagic vesicles or ER/Golgi compartments; counterstaining with organelle markers can aid interpretation.
• Validate antibody performance in your experimental system, preferably using STX17 knockout or knockdown controls to confirm specificity.
• ELISA application may be useful for quantifying STX17 levels in cell lysates or tissue homogenates; ensure proper antigen coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting research areas such as autophagy and membrane trafficking. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing raw materials for rare targets. Explore our catalog to accelerate your diagnostic development with reliable immunoreagents.