Anti-STX12 Rabbit Polyclonal Antibody for WB and ELISA - Q86Y82

Core Product Specifications and Parameters

Biological Background: Syntaxin-12 Function and Localization

• Syntaxin-12 (STX12) is a SNARE protein that mediates fusion of transport vesicles with target membranes, playing a key role in intracellular trafficking.
• Together with SNARE STX6, STX12 promotes the movement of vesicles from endosomes to the cell membrane, indicating a function in the endocytic recycling pathway.
• STX12 forms complexes with GRIP1, GRIA2, and NSG1 to control the intracellular fate of AMPA receptors (AMPAR) and regulates endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting.
• STX12 is localized to multiple endomembrane compartments, including endosome membrane, Golgi apparatus membrane, early endosome membrane, and recycling endosome membrane.
• The protein is characterized by structural features such as a coiled-coil domain and transmembrane helix, consistent with its role in membrane fusion.
• Post-translational modifications include acetylation and phosphorylation, as indicated by UniProt keywords.
• It is a member of the syntaxin family of SNARE proteins and is involved in protein transport pathways.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein corresponding to amino acids 1-276 of human STX12; the resulting polyclonal antibody likely recognizes multiple epitopes and is suitable for Western Blot (WB) and ELISA applications.
• For WB, a specific band around 32 kDa is expected; consider using appropriate loading controls and validating the antibody in relevant cell or tissue lysates from human, mouse, or rat.
• In ELISA, the polyclonal nature may provide enhanced sensitivity; it is advisable to perform titration experiments to determine the optimal antibody dilution.
• This product has not been validated for other applications such as immunohistochemistry or immunofluorescence; if needed, pilot experiments are recommended.
• Given STX12's involvement in endosomal trafficking and AMPA receptor recycling, this antibody is a useful tool for studies in cell biology and neuroscience.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, providing a wide range of high-quality antibodies, recombinant proteins, and ancillary reagents. This Anti-STX12 Rabbit Polyclonal Antibody supports research in membrane trafficking and neuroscience, and through CamelBio, you can access additional validated antibody pairs, bulk monoclonal/polyclonal antibodies, and custom development services. Let us streamline your procurement process and help accelerate your diagnostic and research projects from concept to clinic.