Anti-STUB1 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q9UNE7

Core Product Specifications and Parameters

Biological Background: STUB1 (CHIP) Function and Localization

• E3 ubiquitin-protein ligase CHIP (STUB1) is a ∼35kDa co-chaperone and RING/U-box type ubiquitin ligase that targets misfolded chaperone substrates for proteasomal degradation, playing a central role in protein quality control. Related references: PMID:10330192, PMID:11146632, PMID:11557750
• Participates in mitochondrial homeostasis by promoting mitophagic removal of dysfunctional mitochondria and protecting against stress-induced apoptosis.
• Highly expressed in skeletal muscle, heart, pancreas, brain, and placenta; also detected in kidney, liver, and lung. Related references: PMID:10330192, PMID:11435423
• Localizes to the cytoplasm, nucleus, and mitochondrion, enabling regulation of diverse substrates across compartments.
• Modulates multiple signaling pathways by ubiquitinating key proteins, including FOXO1 (promoting cardiomyocyte survival), SMAD3 (negatively regulating TGF-β), TP53, RIPK3, and PD-L1. Related references: PMID:19483080, PMID:24613385, PMID:29883609
• Functions as a phosphoprotein; contains TPR repeats for chaperone binding and a U-box domain for ubiquitin transfer; implicated in DNA damage repair, neurodegeneration, and spinocerebellar ataxia.

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length human STUB1 protein (amino acids 1–303), including both the TPR domain and U-box catalytic region, likely recognizing multiple isoforms and post-translationally modified forms.
• For western blotting, a band at ~35kDa is expected; consider using lysates from HeLa, HEK293, or tissues with high expression (e.g., skeletal muscle, heart) as positive controls.
• In IF/ICC, STUB1/CHIP displays cytoplasmic, nuclear, and mitochondrial localization; co-staining with organelle markers is recommended to confirm subcellular distribution.
• The antibody is suitable for immunoprecipitation of native STUB1 complexes; optimize lysis conditions to preserve chaperone interactions and use appropriate negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with streamlined access to validated IVD raw materials. For STUB1/CHIP-focused projects, we supply this rabbit polyclonal antibody along with ancillary reagents and custom development services to support protein quality control and neurodegeneration research. Our one-stop offering includes optimized antibody pairs, bulk reagents, and rare-target sourcing to accelerate your assay development from concept to clinic.