Anti-STT3B Rabbit Polyclonal Antibody for WB, ELISA - Q8TCJ2

Core Product Specifications and Parameters

Biological Background: STT3B Function and Localization

• STT3B acts as the catalytic subunit of the OST complex, catalyzing the initial transfer of a glycan (Glc3Man9GlcNAc2) from dolichol-pyrophosphate to Asn-X-Ser/Thr motifs, a crucial step in protein N-glycosylation. Related references: PMID:19167329, PMID:31296534, PMID:31831667
• STT3B-containing complexes mediate both co- and post-translational N-glycosylation; they are essential for efficient post-translational modification and can rescue skipped glycosylation sites. Related references: PMID:19167329, PMID:22607976, PMID:31296534
• In the ERAD pathway, STT3B N-glycosylates misfolded or unfolded proteins, marking them for ubiquitin-dependent degradation. Related references: PMID:19167329, PMID:22607976
• Specifically glycosylates the disease variant AMYL-TTR ('Asp-38') at Asn-118, leading to its degradation. Related references: PMID:19167329, PMID:22607976
• Localized to the endoplasmic reticulum membrane as a multi-pass transmembrane protein.
• Expressed in heart, brain, placenta, lung, liver, muscle, kidney, and pancreas; detected in skin fibroblasts at the protein level.
• Post-translationally modified by acetylation and phosphorylation; binds metal cofactors (Mg2+, Mn2+). Keywords include congenital disorder of glycosylation, glycoprotein, glycosyltransferase.

Experimental Guidance and Technical Tips

• The immunogen targets the N-terminal region (amino acids 1-100), which is conserved between human and mouse, supporting the observed cross-reactivity. Validate in your specific sample matrix.
• For Western blotting, expect a band at approximately 94 kDa. Optimize antibody dilution and we recommend using enriched ER fractions to enhance detection of endogenous STT3B.
• In ELISA, consider using the recombinant immunogen as a positive control. Titrate antibody concentration to achieve optimal signal-to-noise ratio.
• Always include appropriate positive and negative controls to confirm specificity, especially when analyzing low-abundance targets.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research teams a streamlined, one-stop sourcing solution for high-quality IVD raw materials. This anti-STT3B rabbit polyclonal antibody is part of our comprehensive portfolio for N-glycosylation and ERAD research, complemented by validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Leverage our expertise to source rare targets or customize production, supporting your projects from concept to clinical application.