Anti-STRN3 Polyclonal Antibody for WB - Q13033

Core Product Specifications and Parameters

Biological Background: STRN3 Function and Localization

• STRN3 (also known as SG2NA, S/G2 antigen) is a calmodulin-binding scaffolding protein that serves as the core component of striatin-interacting phosphatase and kinase (STRIPAK) complexes (Probable). Related references: PMID:18782753 PMID:30622739 PMID:33633399
• STRIPAK complexes regulate multiple signaling pathways including Hippo and MAPK, controlling critical processes such as cell growth, differentiation, and apoptosis.
• STRN3 localizes predominantly to the cytoplasm and membrane, consistent with its role as a signaling scaffold.
• Post-translational modifications, including acetylation and phosphorylation, modulate STRN3 activity and protein interactions.
• The protein contains WD repeats and a coiled-coil domain, which facilitate assembly of the STRIPAK complex.
• Multiple isoforms of STRN3 are generated through alternative splicing, potentially diversifying STRIPAK function.
• Originally identified as a cell cycle autoantigen (SG2NA), STRN3 exhibits elevated expression during S and G2 phases.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 200–460, targeting the central region of human STRN3; this region is conserved across isoforms, potentially enabling detection of multiple splice variants.
• For Western blotting, expect a band at approximately 87 kDa; validate in relevant cell lines and use appropriate loading controls.
• For immunohistochemistry (IHC-P), optimize antigen retrieval conditions and include both positive (e.g., human cancer tissue) and negative controls.
• For immunofluorescence (IF/ICC), permeabilization is recommended to access the cytoplasmic and membrane-localized target; counterstaining with DAPI can confirm cellular localization.
• Cross-reactivity with mouse and rat is reported; however, verify species-specific reactivity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As a trusted partner in the life science supply chain, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This anti-STRN3 polyclonal antibody supports research into STRIPAK complex signaling, cell cycle regulation, and cancer biology.