Anti-STRIP1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q5VSL9

Core Product Specifications and Parameters

Biological Background: STRIP1 Function and Localization

• STRIP1 (Striatin-interacting protein 1), also known as FAM40A or FAR11A, is a core component of the striatin-interacting phosphatase and kinase (STRIPAK) complexes, which are key regulators of cellular signaling.
• It plays a pivotal role in cell morphology and cytoskeletal organization by modulating cortical actin filament dynamics and maintaining cell shape.
• The STRIPAK complex, which includes STRIP1, governs multiple signaling pathways, including Hippo, MAPK, and nuclear receptor signaling, thereby influencing processes like cell growth, differentiation, and apoptosis.
• STRIP1 is predominantly localized in the cytoplasm, as indicated by subcellular localization data.
• Post-translational modifications such as acetylation and phosphorylation, along with alternative splicing, contribute to its functional diversity (UniProt keywords: Acetylation, Phosphoprotein, Alternative splicing).
• The protein has been identified through proteomic approaches and its three-dimensional structure has been partially resolved (UniProt entry Q5VSL9).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 743-837 of human STRIP1, a region near the C-terminus; this design may be suitable for detecting both full-length and certain truncated isoforms.
• For Western blot, consider using whole cell lysates from human, mouse, or rat cells, given the validated cross-reactivity. Expect a band near 96 kDa; optimize loading controls and detection conditions.
• In immunofluorescence (IF/ICC), STRIP1 is expected to exhibit cytoplasmic staining; appropriate fixation and permeabilization conditions should be validated in the relevant cell system.
• For ELISA applications, titer and specificity should be validated using recombinant STRIP1 protein or peptide to ensure reliable quantitative results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a comprehensive one-stop sourcing solution for IVD raw materials, covering every stage from concept to clinic. This anti-STRIP1 rabbit polyclonal antibody supports research into STRIPAK complex biology, cytoskeletal dynamics, and cell signaling pathways. From validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target raw-material sourcing, CamelBio empowers your assay development with reliable and efficient supply chains.