Anti-STRBP Polyclonal Antibody for WB and ELISA - Q96SI9

Core Product Specifications and Parameters

Biological Background: STRBP Function and Localization

• STRBP encodes the spermatid perinuclear RNA-binding protein, also known as SPNR, ILF3L, or HEL162.
• It is implicated in spermatogenesis and sperm function, playing a role in male germ cell development and fertility.
• The protein binds double-stranded DNA and RNA, with highest affinity for poly(I:C) RNA, and also interacts with single-stranded poly(G) RNA and specific mRNA 3'-UTRs (e.g., PRM1).
• STRBP localizes predominantly to the cytoplasm, consistent with its post-transcriptional regulatory functions.
• It contains repeated dsRNA-binding domains and undergoes methylation, which may modulate its activity or interactions.
• Alternative splicing gives rise to multiple protein isoforms, potentially diversifying its functional roles in different tissues or developmental stages.
• UniProt keywords associate STRBP with spermatogenesis, cell differentiation, and DNA/RNA metabolism.

Experimental Guidance and Technical Tips

• The immunogen targets the N-terminal region (aa 40-190), upstream of the predicted dsRNA-binding domains; thus, the antibody may recognize multiple isoforms and avoid interference with RNA-binding functions.
• For western blot, the predicted molecular weight is approximately 74 kDa; validate band specificity using appropriate positive (e.g., testis lysate) and negative controls.
• ELISA applications may benefit from optimization of coating antigen and antibody dilution, as no standardized protocol is provided.
• While only human cross-reactivity is confirmed, this antibody may react with orthologs in other species; verify empirically if needed.
• Consider co-immunofluorescence or immunohistochemistry with spermatid markers to confirm spatial expression in testis sections, noting the cytoplasmic localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw-material sourcing, offering this anti-STRBP polyclonal antibody along with validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents tailored to diagnostic assay development. Our expertise covers rare and specialized targets like STRBP, supporting researchers and manufacturers from discovery through to clinical applications. Leverage our one-stop supply chain to streamline your spermatogenesis-related IVD projects.