Anti-STRAP Rabbit Polyclonal Antibody for WB - Q9Y3F4

Core Product Specifications and Parameters

Biological Background: STRAP Function and Localization

• STRAP (serine-threonine kinase receptor-associated protein), also known as MAWD, UNRIP, or PT-WD, is a 38 kDa WD-repeat protein encoded by the STRAP gene on human chromosome 12.
• It functions as an essential component of the SMN complex, which catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, thereby playing a critical role in pre-mRNA splicing.
• STRAP facilitates the transfer of Sm proteins from the pICln-Sm complex to the SMN complex during core snRNP assembly and regulates the cellular distribution of the SMN complex.
• Beyond its role in splicing, STRAP negatively regulates TGF-beta signaling and positively modulates PDPK1 kinase activity by enhancing autophosphorylation and reducing 14-3-3 binding.
• The protein localizes to both the cytoplasm and the nucleus, consistent with its involvement in snRNP assembly (cytoplasm) and splicing events (nucleus).
• STRAP contains multiple WD repeats, which mediate protein-protein interactions critical for its scaffold and regulatory functions.
• According to UniProt, STRAP is subject to alternative splicing and phosphorylation, which may modulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-350 of human STRAP, encompassing the full-length protein; thus, this polyclonal antibody may recognize multiple epitopes, offering robust detection across applications.
• For Western blotting, a band around 38 kDa is expected; verify band specificity using lysates from STRAP-knockout cell lines or tissues.
• In immunofluorescence and immunocytochemistry, anticipate cytoplasmic and nuclear staining; validate using appropriate subcellular markers.
• For immunoprecipitation, this antibody may capture endogenous STRAP and its interacting partners; optimize antibody concentration and incubation conditions for your specific sample.
• This antibody has been validated with human, mouse, and rat samples; for other species, check sequence homology before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio of validated antibodies, antigens, and ancillary reagents to support diagnostic development from concept to clinic. This STRAP polyclonal antibody exemplifies our commitment to providing high-quality, knockout-validated tools for research on mRNA splicing and TGF-beta signaling. Beyond individual reagents, CamelBio can source bulk quantities, develop custom antibody pairs, and supply rare target raw materials to meet your assay development needs.