Anti-STRAP Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y3F4

Core Product Specifications and Parameters

Biological Background: STRAP Function and Localization

• STRAP (Serine-threonine kinase receptor-associated protein), also known as MAWD, UNRIP, and PT-WD, is a WD-40 repeat-containing protein that functions as a component of the SMN complex, which is crucial for the assembly of small nuclear ribonucleoproteins (snRNPs) and pre-mRNA splicing.
• The SMN complex catalyzes the formation of the core snRNP by facilitating the transfer of Sm proteins from the pICln-Sm complex onto the small nuclear RNA, a process in which STRAP participates by influencing the cellular distribution of the SMN complex.
• STRAP negatively regulates TGF-beta signaling, providing a link between spliceosomal functions and cell signaling pathways.
• Additionally, STRAP positively regulates PDPK1 kinase activity by enhancing its autophosphorylation and reducing its association with 14-3-3 proteins, thereby promoting downstream signaling.
• Subcellularly, STRAP is localized in both the cytoplasm and nucleus, consistent with its roles in snRNP assembly and signaling modulation.
• The protein is subject to alternative splicing and is identified as a phosphoprotein in proteomics studies, with a molecular weight of approximately 38 kDa.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein covering amino acids 1-350 of human STRAP, which corresponds to nearly the full-length protein. This design may facilitate the recognition of both native and denatured forms of STRAP in various applications.
• For Western blot (WB) analysis, consider using a standard denaturing gel and transferring to a membrane; the predicted molecular weight is 38 kDa, but post-translational modifications may cause shifts. Validate the antibody in the relevant cell or tissue lysates.
• In immunofluorescence/immunocytochemistry (IF/ICC), ensure appropriate fixation and permeabilization protocols to detect STRAP in its cytoplasmic and nuclear locales.
• This antibody is cross-reactive with human, mouse, and rat STRAP, making it suitable for comparative studies across species. However, always confirm reactivity in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with comprehensive access to IVD raw materials, technical services, and consulting—from concept to clinic. As a trusted partner, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw material sourcing. For researchers studying spliceosome assembly and TGF-beta/PDPK1 signaling, we offer this high-quality STRAP antibody alongside tailored procurement solutions to accelerate your diagnostic development and life science projects.