Anti-STRADA Rabbit Polyclonal Antibody for WB, ELISA - Q7RTN6

Core Product Specifications and Parameters

Biological Background: STRADA Function and Localization

• STRADA (STE20-related kinase adapter protein alpha), also known as LYK5, STRAD, and NY-BR-96, is a pseudokinase that acts as an essential adapter for the STK11/LKB1 tumor suppressor kinase.
• In complex with CAB39/MO25, STRADA binds to STK11/LKB1 and promotes its active conformation, thereby regulating LKB1 downstream signaling pathways involved in cell cycle control and energy metabolism.
• Encoded by the STRADA gene on chromosome 17, alternative splicing generates multiple transcript variants; the immunogen corresponds to amino acids 25-210 of isoform 2 (NP_699166.2).
• Subcellularly, STRADA localizes to both the nucleus and cytoplasm, consistent with its role in shuttling STK11/LKB1 between compartments.
• Key post-translational modifications include phosphorylation; the protein contains an ATP-binding domain typical of active kinases despite its pseudokinase classification.
• Keywords associated with STRADA include cell cycle, chromosomal rearrangement, and phosphoprotein, highlighting its relevance to genomic stability and cancer biology.

Experimental Guidance and Technical Tips

• The immunogen was a recombinant fusion protein spanning amino acids 25-210 of human STRADA (isoform 2), which is conserved in mouse; thus, the antibody is expected to detect both human and mouse STRADA.
• For Western blotting, consider testing a range of dilutions (e.g., 1:500–1:2000) and validate in lysates from relevant cell lines or tissues expressing STRADA.
• For ELISA, the antibody can be used as a capture or detection reagent; pair with appropriate secondary antibodies and optimize coating conditions.
• Since STRADA functions as an adapter in the LKB1 complex, co-immunoprecipitation or pull-down assays may also be compatible, though not explicitly validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers and research laboratories with a comprehensive sourcing platform for high-quality raw materials. This anti-STRADA polyclonal antibody supports studies on LKB1/STRADA signaling and cell cycle regulation, and CamelBio can assist with sourcing complementary reagents such as recombinant STK11/LKB1 proteins, MO25 antibodies, and matched antibody pairs for assay development. Leverage our network to streamline your procurement process from discovery to clinical applications.