Anti-STK3/MST2 (phospho T117) Polyclonal Antibody for WB and ELISA - Q13188

Core Product Specifications and Parameters

Biological Background: STK3/MST2 Function and Localization

• STK3/MST2 (Serine/threonine-protein kinase 3), also known as Krs-1 or MST2, is a core kinase of the Hippo signaling pathway that controls organ size and tumor suppression by restricting proliferation and promoting apoptosis. Related references: PMID:15688006, PMID:16930133, PMID:23972470
• Upon activation and caspase-cleavage, STK3/MST2 translocates to the nucleus and induces chromatin condensation and DNA fragmentation, playing a key role in stress‑induced apoptosis. Related references: PMID:11278283, PMID:8566796, PMID:8816758
• In the Hippo kinase cascade, STK3/MST2, in complex with SAV1, phosphorylates and activates LATS1/2, which in turn phosphorylates and inactivates the oncoproteins YAP1 and TAZ. Related references: PMID:15688006, PMID:16930133, PMID:23972470
• The protein localizes to the cytoplasm, nucleus, and centrosomes, reflecting its dynamic roles in signal transduction and cell cycle regulation.
• STK3/MST2 phosphorylates NEK2 at centrosomes to regulate centrosome disjunction, and also targets MOBKL1A/B, RASSF2, and RAF1 to modulate proliferation and differentiation. Related references: PMID:21076410, PMID:21723128, PMID:19525978, PMID:20212043, PMID:18328708, PMID:18362890
• The kinase is highly expressed in adult kidney, skeletal muscle, and placenta, with low expression in heart, lung, and brain, and its activity is regulated by acetylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• This antibody is raised against a synthetic phospho-peptide corresponding to Thr117 of human STK3/MST2; it is designed to specifically recognize the phosphorylated form at this site.
• For Western blot, consider using phosphatase-treated lysates or known stimuli that induce MST2 phosphorylation as negative and positive controls.
• The antibody is qualified for WB and ELISA, and has been shown to cross-react with mouse and rat endogenous STK3/MST2; however, validation in the specific sample system is recommended.
• Due to the antibody’s phospho-specificity, blocking with phospho-peptide or treatment with λ‑phosphatase can confirm signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one‑stop sourcing partner for IVD raw materials, offering validated research‑grade antibodies, antibody pairs, and ancillary reagents to support your diagnostic and translational projects. Our portfolio includes phosphorylated‑specific antibodies like this anti‑STK3/MST2 (phospho T117) product, which targets the apoptosis and Hippo signaling network—key areas for oncology and cell‑biology research. From bulk monoclonal/polyclonal supplies to rare target sourcing, we help bridge the gap between concept and clinical application.