Anti-STK3/MST2 Monoclonal Antibody for WB, IF/ICC, ELISA - Q13188

Core Product Specifications and Parameters

Biological Background: STK3/MST2 Function and Localization

• STK3/MST2 is a stress-activated, pro-apoptotic serine/threonine-protein kinase that, after caspase cleavage, translocates to the nucleus and triggers chromatin condensation and DNA fragmentation. Related references: PMID:11278283, PMID:8566796, PMID:8816758

• It is a core component of the Hippo signaling pathway, forming a complex with SAV1 to phosphorylate and activate LATS1/2, which in turn phosphorylates and inactivates YAP1 and WWTR1/TAZ, thereby restricting proliferation and promoting apoptosis. Related references: PMID:15688006, PMID:16930133, PMID:23972470

• Phosphorylates NEK2 and is involved in centrosome disjunction by regulating NEK2 localization to the centrosome and subsequent phosphorylation of CROCC and CEP250. Related references: PMID:21076410, PMID:21723128

• In conjunction with SAV1, STK3/MST2 modulates ESR1 transcriptional activity by affecting its phosphorylation. Related references: PMID:21104395

• Positively regulates RAF1 activation by suppressing inhibitory phosphorylation on Ser-259. Related references: PMID:20212043

• Localizes to the cytoplasm, nucleus, and centrosome; expressed at high levels in adult kidney, skeletal muscle, and placenta tissues, and at very low levels in adult heart, lung, and brain tissues.

• Post-translationally modified by acetylation and ubiquitination; classified as a serine/threonine-protein kinase involved in apoptosis, and contains ATP-binding, coiled coil, and nucleotide-binding domains.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to the N-terminal region (amino acids 1-100) of human STK3/MST2. When using this antibody on mouse samples, validate specificity as cross-reactivity is confirmed but sequence homology may vary slightly.
• This antibody is suitable for Western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA. For WB, consider using whole-cell lysates from human or mouse cell lines known to express STK3/MST2, and ensure proper blocking and antibody dilution optimization.
• For IF/ICC, fixation and permeabilization conditions should be optimized to detect nuclear and cytoplasmic pools of STK3/MST2. As STK3/MST2 can translocate to the nucleus upon caspase cleavage, monitoring apoptotic stimuli may enhance nuclear signal.
• Given its role in centrosome disjunction, this antibody may also be useful for centrosome studies; consider appropriate cell synchronization protocols and high-resolution microscopy.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material sourcing partner that supports diagnostic manufacturers and research laboratories from concept to clinical application. With this high-quality anti-STK3/MST2 monoclonal antibody, we provide a reliable tool for apoptosis and Hippo signaling pathway research. Our portfolio includes validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, ensuring stable supply and technical support for your assay development needs.