Anti-STK39 Rabbit Polyclonal Antibody for WB and ELISA - Q9UEW8

Core Product Specifications and Parameters

Biological Background: STK39 Function and Localization

• STK39 (STE20/SPS1-related proline-alanine-rich protein kinase) is an effector serine/threonine-protein kinase component of the WNK-SPAK/OSR1 kinase cascade involved in ion transport, response to hypertonic stress, and blood pressure regulation. Related references: PMID:16669787, PMID:18270262, PMID:21321328
• It specifically recognizes and binds proteins with an RFXV motif and acts downstream of WNK kinases (WNK1-4) to phosphorylate ion cotransporters such as SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A5/KCC2, and SLC12A6/KCC3, thereby regulating their activity. Related references: PMID:16669787, PMID:21321328
• Mediates regulatory volume increase in response to hyperosmotic stress by catalyzing phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1, and SLC12A6/KCC3 downstream of WNK1 and WNK3. Related references: PMID:12740379, PMID:16669787, PMID:21321328
• Phosphorylates Na-K-Cl cotransporters SLC12A1 and SLC12A2 to promote activation and ion influx, while simultaneously inhibiting K-Cl cotransporters SLC12A5 and SLC12A6 to block ion efflux. Related references: PMID:16669787, PMID:19665974, PMID:21321328
• Acts as a key regulator of NaCl reabsorption in the distal nephron by mediating phosphorylation and activation of the thiazide-sensitive Na-Cl cotransporter SLC12A3/NCC downstream of WNK4. Related references: PMID:18270262
• Subcellular localization is predominantly cytoplasmic and nuclear.
• Predominantly expressed in brain and pancreas, with lower levels in heart, lung, kidney, skeletal muscle, liver, placenta, and testis.
• The protein undergoes acetylation and alternative splicing, and is classified as an ATP-binding phosphoprotein kinase.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide from amino acids 500–600 of human STK39, targeting the C-terminal region. Peptide competition assays may be considered to confirm binding specificity.
• For Western blot, the predicted molecular weight is 59 kDa. Validate the antibody in your specific sample system, as post-translational modifications or alternative splice variants can produce additional bands.
• In ELISA, optimization of coating antigen concentration and detection conditions is recommended to achieve optimal signal-to-noise ratios.
• As a polyclonal antibody, it may recognize multiple epitopes, potentially offering robust detection in both denaturing and native conditions. Assess lot-to-lot consistency for critical applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets like STK39, a central kinase in ion transport and blood pressure pathways, CamelBio can also assist in sourcing rare-target raw materials and providing technical consulting to accelerate your diagnostic development from concept to clinic.