Anti-STK39 Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q9UEW8

Core Product Specifications and Parameters

Biological Background: STK39 Function and Localization

• STK39 (Serine/threonine-protein kinase 39), also known as SPAK or DCHT, is a 59 kDa effector kinase of the STE20 family with proline-alanine-rich regions.
• It functions as a key component of the WNK-SPAK/OSR1 kinase cascade, phosphorylating ion cotransporters such as SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A5/KCC2, and SLC12A6/KCC3 to regulate their activities. Related references: PMID:16669787, PMID:21321328
• STK39 mediates regulatory volume increase by simultaneously activating Na-K-Cl cotransporters (NKCC1, NKCC2) and inhibiting K-Cl cotransporters (KCC2, KCC3) downstream of WNK1 and WNK3 kinases. Related references: PMID:16669787, PMID:19665974, PMID:21321328
• In the kidney, it specifically phosphorylates and activates the thiazide-sensitive Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells, contributing to NaCl reabsorption and blood pressure regulation. Related references: PMID:18270262
• The kinase recognizes and binds substrates containing an RFXV motif, crucial for targeting ion cotransporters. Related references: PMID:16669787, PMID:21321328
• STK39 localizes to both the cytoplasm and nucleus, and is predominantly expressed in the brain and pancreas, with additional expression in heart, lung, kidney, and other tissues.
• Post-translational modifications include acetylation and phosphorylation, and the gene undergoes alternative splicing, producing multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 401–545). For Western blot, a band near 59 kDa is expected; validate with positive and negative controls in your sample system.
• For immunofluorescence and immunohistochemistry, consider that STK39 can be found in both cytoplasm and nucleus; optimize permeabilization and fixation protocols accordingly.
• This antibody shows cross-reactivity with mouse and rat STK39, making it suitable for cross-species studies; however, confirm reactivity in your model if not yet tested.
• Since STK39 is a kinase involved in ion transport, consider using appropriate inhibitors or activators of the WNK-SPAK/OSR1 cascade to modulate its activity as experimental controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined, one-stop sourcing solution for IVD raw materials, from validated antibody pairs to optimized monoclonal/polyclonal antibodies like this STK39 Rabbit mAb, along with bulk ancillary reagents and custom rare-target sourcing. Whether you are developing assays for hypertension research, renal function studies, or ion transport pathways, our supply network can support your project from early development to clinical application. We help diagnostic manufacturers and labs bridge the gap between concept and clinic with reliable, high-quality raw materials.