Anti-STK38L Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y2H1

Core Product Specifications and Parameters

Biological Background: STK38L Function and Localization

• STK38L is a serine/threonine-protein kinase, also known as NDR2 (Nuclear Dbf2-related kinase 2), belonging to the NDR/LATS family of kinases.
• The kinase is involved in the regulation of structural processes critical for neuronal differentiation and the maintenance of mature neuronal cells.
• STK38L is predominantly localized in the cytoplasm, associated with the cytoskeleton, and also found at the membrane, reflecting its roles in cell structure and signaling.
• Ubiquitously expressed with the highest levels in the thymus, indicating potential functions in immune as well as neuronal tissues.
• Key functional features include ATP-binding, magnesium-dependent kinase activity, actin-binding, and serine/threonine phosphorylation, as annotated in UniProt.
• Post-translational modifications, including acetylation and phosphorylation, have been identified through proteomics studies, which may regulate its activity and interactions.
• Multiple isoforms are produced by alternative splicing, though their distinct functional roles are not yet fully characterized.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 165-464 of human STK38L, targeting a central region of the protein; consider sequence alignment if intending to use with alternative splice variants or other species variants beyond those listed.
• For Western blot (WB), the expected molecular weight is approximately 54 kDa; however, post-translational modifications may cause slight shifts—validate in the relevant sample system.
• In immunofluorescence/immunocytochemistry (IF/ICC), expect staining primarily in the cytoplasm and cytoskeletal compartments; appropriate fixation and permeabilization methods should be optimized to preserve both antigenicity and cell architecture.
• For ELISA applications, the polyclonal nature may enhance sensitivity; consider verifying detection limits and specificity in the intended sample matrix.
• Cross-reactivity with mouse and rat has been indicated; when working with these species, confirm performance under your specific experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibodies, bulk ancillary reagents, and specialized support for rare target sourcing. For researchers and manufacturers investigating STK38L in neuronal signaling or cellular differentiation, our portfolio includes reliable polyclonal antibodies and customizable procurement solutions. Let CamelBio streamline your supply chain so you can focus on advancing diagnostic development with confidence.